Spacer-Based Selectivity in the Binding of “Two-Prong” Ligands to Recombinant Human Carbonic Anhydrase I

• Published in: Biochemistry (Easton) Vol. 44; no. 9; pp. 3211 - 3224
• Main Authors: Banerjee, Abir L, Eiler, Daniel, Roy, Bidhan C, Jia, Xiao, Haldar, Manas K, Mallik, Sanku, Srivastava, D. K
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 08.03.2005
• Subjects: Acetazolamide - chemistry; Acetazolamide - metabolism; Benzenesulfonamides; Binding, Competitive; Calorimetry; Carbonic Anhydrase I - antagonists & inhibitors; Carbonic Anhydrase I - metabolism; Carbonic Anhydrase Inhibitors - chemistry; Carbonic Anhydrase Inhibitors - metabolism; Dansyl Compounds - metabolism; Diethyl Pyrocarbonate - chemistry; Humans; Imino Acids - chemical synthesis; Imino Acids - metabolism; Kinetics; Ligands; Protein Binding; Recombinant Proteins - antagonists & inhibitors; Recombinant Proteins - metabolism; Spectrometry, Fluorescence; Sulfonamides - chemical synthesis; Sulfonamides - metabolism; Titrimetry

Benzenesulfonamide and iminodiacetate (IDA)-conjugated Cu2+ independently interact at the active site and a peripheral site of carbonic anhydrases, respectively [Banerjee, A. L., Swanson, M., Roy, B. C., Jia, X., Haldar, M. K., Mallik, S., and Srivastava, D. K. (2004) J. Am. Chem. Soc. 126, 10875−10...