Role of Hydrophobic Interactions in the Encounter Complex Formation of the Plastocyanin and Cytochrome f Complex Revealed by Paramagnetic NMR Spectroscopy

• Published in: Journal of the American Chemical Society Vol. 135; no. 20; pp. 7681 - 7692
• Main Authors: Scanu, Sandra, Foerster, Johannes M, Ullmann, G. Matthias, Ubbink, Marcellus
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 22.05.2013
• Subjects: Cytochromes f - chemistry; Cytochromes f - isolation & purification; Cytochromes f - metabolism; Hydrophobic and Hydrophilic Interactions; Models, Molecular; Monte Carlo Method; Nuclear Magnetic Resonance, Biomolecular; Plastocyanin - chemistry; Plastocyanin - metabolism

Protein complex formation is thought to be at least a two-step process, in which the active complex is preceded by the formation of an encounter complex. The interactions in the encounter complex are usually dominated by electrostatic forces, whereas the active complex is also stabilized by noncoval...