Incorporation of a complete set of deoxyadenosine and thymidine analogs suitable for the study of protein nucleic acid interactions into oligodeoxynucleotides. Application to the EcoRV restriction endonuclease and modification methylase

• Published in: Biochemistry (Easton) Vol. 29; no. 42; pp. 9891 - 9901
• Main Authors: Newman, Patrick C, Nwosu, Victor U, Williams, David M, Cosstick, Richard, Seela, Frank, Connolly, Bernard A
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.10.1990
• Subjects: Adenosine - analogs & derivatives; Adenosine - chemical synthesis; Base Sequence; Biological and medical sciences; Deoxyadenosines - chemical synthesis; Deoxyadenosines - metabolism; Deoxyribonucleases, Type II Site-Specific - metabolism; DNA-Binding Proteins - metabolism; Fundamental and applied biological sciences. Psychology; Interactions. Associations; Intermolecular phenomena; Molecular biophysics; Molecular Conformation; Molecular Sequence Data; Oligodeoxyribonucleotides - chemical synthesis; Oligodeoxyribonucleotides - metabolism; Protein Binding; Site-Specific DNA-Methyltransferase (Adenine-Specific) - metabolism; Structure-Activity Relationship; Substrate Specificity; Thymidine - analogs & derivatives; Thymidine - chemical synthesis; Enzyme; Escherichia coli; DNA (cytosine-5-)-methyltransferase; HPLC chromatography; Molecular interaction; Enzymatic activity; Analog; DNA; Circular dichroism spectrometry; Restriction endonuclease; Nucleotide; Bacteria; Oligonucleotide; Kinetics; Comparative study; Enterobacteriaceae

A complete set of dA and T analogues designed for the study of protein DNA interactions has been prepared. These modified bases have been designed by considering the groups on the dA and T bases that are accessible to proteins when these bases are incorporated into double-helical B-DNA [Seeman, N. C...