Effects of Cell Culture Media on the Dynamic Formation of Protein−Nanoparticle Complexes and Influence on the Cellular Response

• Published in: ACS nano Vol. 4; no. 12; pp. 7481 - 7491
• Main Authors: Maiorano, Gabriele, Sabella, Stefania, Sorce, Barbara, Brunetti, Virgilio, Malvindi, Maria Ada, Cingolani, Roberto, Pompa, Pier Paolo
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 28.12.2010
• Subjects: Animals; Cattle; Cell Culture Techniques - methods; Cell Line, Tumor; Cell Proliferation - drug effects; Citrates - chemistry; Culture Media - pharmacology; Gold - chemistry; Gold - metabolism; Gold - toxicity; Humans; Metal Nanoparticles - chemistry; Nanoparticles - chemistry; Nanoparticles - toxicity; Particle Size; Protein Binding - drug effects; Proteins - metabolism; nanobiointeractions; nanotoxicity; gold nanoparticles; in vitro studies; protein corona

The development of appropriate in vitro protocols to assess the potential toxicity of the ever expanding range of nanoparticles represents a challenging issue, because of the rapid changes of their intrinsic physicochemical properties (size, shape, reactivity, surface area, etc.) upon dispersion in...