Hydroxylation of Camphor by Reduced Oxy-Cytochrome P450cam:  Mechanistic Implications of EPR and ENDOR Studies of Catalytic Intermediates in Native and Mutant Enzymes

• Published in: Journal of the American Chemical Society Vol. 123; no. 7; pp. 1403 - 1415
• Main Authors: Davydov, Roman, Makris, Thomas M, Kofman, Victoria, Werst, David E, Sligar, Stephen G, Hoffman, Brian M
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 21.02.2001
• Subjects: Camphor - metabolism; Camphor 5-Monooxygenase - metabolism; Catalysis; Electron Spin Resonance Spectroscopy; Freezing; Hydroxylation

We have employed γ-irradiation at cryogenic temperatures (77 K and also ∼6 K) of the ternary complexes of camphor, dioxygen, and ferro-cytochrome P450cam to inject the “second” electron of the catalytic process. We have used EPR and ENDOR spectroscopies to characterize the primary product of reducti...