Site-Specific Characterization of HIV-1 Nucleocapsid Protein Binding to Oligonucleotides with Two Binding Sites

• Published in: Biochemistry (Easton) Vol. 48; no. 11; pp. 2422 - 2430
• Main Authors: Avilov, Sergiy V, Godet, Julien, Piémont, Etienne, Mély, Yves
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 24.03.2009
• Subjects: 2-Aminopurine - chemistry; Amino Acid Motifs; Amino Acid Sequence; Binding Sites; Biochemistry, Molecular Biology; Biophysics; gag Gene Products, Human Immunodeficiency Virus - chemistry; HIV-1 - chemistry; Human immunodeficiency virus 1; Kinetics; Life Sciences; Molecular Sequence Data; Oligonucleotides - chemistry; Protein Binding

The nucleocapsid protein (NC) of HIV-1 is a highly conserved protein essential for the virus life cycle that constitutes an attractive target for new antiviral agents. Most NC functions rely on its binding to the HIV-1 genomic RNA and its DNA copies that contain multiple and possibly interdependent...