Activity-Based Protein Profiling in Vivo Using a Copper(I)-Catalyzed Azide-Alkyne [3 + 2] Cycloaddition

• Published in: Journal of the American Chemical Society Vol. 125; no. 16; pp. 4686 - 4687
• Main Authors: Speers, Anna E, Adam, Gregory C, Cravatt, Benjamin F
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 23.04.2003
• Subjects: Aldehyde Dehydrogenase - analysis; Aldehyde Dehydrogenase 1 Family; Alkynes - chemistry; Analytical chemistry; Animals; Azides - chemistry; Breast Neoplasms - enzymology; Catalysis; Chemistry; Chlorocebus aethiops; Copper - chemistry; COS Cells; Exact sciences and technology; Glutathione Transferase - analysis; Humans; Isoenzymes - analysis; Liver - enzymology; Mice; Miscellaneous; Proteomics - methods; Retinal Dehydrogenase; Structure-Activity Relationship; Transfection; Tumor Cells, Cultured

Toward the goal of assigning function to the tens of thousands of protein products encoded by eukaryotic and prokaryotic genomes, the field of proteomics requires new technologies that can functionally characterize proteins within the dynamic environment of the cell, where these biomolecules are sub...