Optimized Enzyme-Linked Immunosorbent Assay for Anti-PEG Antibody Detection in Healthy Donors and Patients Treated with PEGylated Liposomal Doxorubicin

• Published in: Molecular pharmaceutics Vol. 21; no. 6; pp. 3053 - 3060
• Main Authors: Li, Yue, Ettah, Utibeabasi, Jacques, Sarah, Gaikwad, Hanmant, Monte, Andrew, Dylla, Layne, Guntupalli, Saketh, Moghimi, S. Moein, Simberg, Dmitri
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 03.06.2024
• Subjects: Adult; Doxorubicin - analogs & derivatives; Doxorubicin - therapeutic use; Enzyme-Linked Immunosorbent Assay; Female; Humans; Immunoglobulin G - blood; Immunoglobulin G - immunology; Immunoglobulin M - blood; Immunoglobulin M - immunology; Liposomes; Male; Middle Aged; Neoplasms - drug therapy; Neoplasms - immunology; Polyethylene Glycols - therapeutic use; PEGylated liposomal doxorubicin; Doxil; PEG; specific; patients; ELISA

There is considerable interest in quantifying anti-PEG antibodies, given their potential involvement in accelerated clearance, complement activation, neutralization, and acute reactions associated with drug delivery systems. Published and commercially available anti-PEG enzyme-linked immunosorbent a...