Domain-Swapped Dimeric Structure of a Stable and Functional De Novo Four-Helix Bundle Protein, WA20

• Published in: The journal of physical chemistry. B Vol. 116; no. 23; pp. 6789 - 6797
• Main Authors: Arai, Ryoichi, Kobayashi, Naoya, Kimura, Akiho, Sato, Takaaki, Matsuo, Kyoko, Wang, Anna F, Platt, Jesse M, Bradley, Luke H, Hecht, Michael H
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 14.06.2012
• Subjects: Bundles; Bundling; Crystal structure; Dispersions; Libraries; Models, Molecular; Monomers; Protein Folding; Protein Stability; Protein Structure, Secondary; Proteins; Proteins - chemistry; Proteins - genetics; Proteins - isolation & purification; Scattering, Small Angle; Temperature; Three dimensional; X-Ray Diffraction

To probe the potential for activity in unevolved amino acid sequence space, we created a third generation combinatorial library of de novo four-helix bundle proteins. The “artificial superfamily” of helical bundles was designed using binary patterning of polar and nonpolar residues, and expressed in...