High control coefficient of transketolase in the nonoxidative pentose phosphate pathway of human erythrocytes: NMR, antibody, and computer simulation studies

• Published in: Biochemistry (Easton) Vol. 31; no. 51; pp. 12792 - 12798
• Main Authors: Berthon, Hilary A, Kuchel, Philip W, Nixon, Peter F
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 29.12.1992
• Subjects: Biological and medical sciences; Blotting, Western; Cell metabolism, cell oxidation; Cell physiology; Computer Simulation; erythrocytes; Erythrocytes - enzymology; Fundamental and applied biological sciences. Psychology; Humans; Immune Sera; Kinetics; Luminescent Measurements; Magnetic Resonance Spectroscopy; man; mathematical models; Molecular and cellular biology; N.M.R; Pentose Phosphate Pathway; Pentosephosphates - blood; Ribosemonophosphates - blood; Ribulosephosphates - blood; transketolase; Transketolase - blood; Transketolase - immunology; Human; Regulation(control); Enzymatic activity; Computer simulation; Enzyme; Transketolase; Pentose phosphate pathway; Red blood cell; NMR spectrometry; In vitro; Modeling

The degree of control exerted by transketolase over metabolite flux in the nonoxidative pentose phosphate pathway in human erythrocytes was investigated using transketolase antiserum to modulate the activity of that enzyme. 31P NMR enabled the simultaneous measurement of the levels of pentose phosph...