Elastin−Calmodulin Scaffold for Protein Microarray Fabrication

• Published in: Langmuir Vol. 23; no. 5; pp. 2277 - 2279
• Main Authors: Jenikova, Gabriela, Lao, U Loi, Gao, Di, Mulchandani, Ashok, Chen, Wilfred
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 27.02.2007
• Subjects: Bacterial Proteins - chemistry; Biophysics - methods; Calcium - chemistry; Calmodulin - chemistry; Chemistry; Chemistry, Physical - methods; Colloidal state and disperse state; Edetic Acid - chemistry; Elastin - chemistry; Exact sciences and technology; General and physical chemistry; Luminescent Proteins - chemistry; Microscopy, Fluorescence; Oligonucleotide Array Sequence Analysis; Protein Array Analysis - methods; Proteins - chemistry; Recombinant Proteins - chemistry; Surface physical chemistry; Temperature; Incorporation; Association; Purification; Calcium; Immobilization; EDTA; Orientation; Protein

In this work, we report a new method to reversibly immobilize proteins to a surface in a functionally active orientation directly from cell lysate by employing a fusion protein consisting of a thermal-responsive elastin (ELP) domain as the surface anchor and a calcium-responsive calmodulin (CalM) do...