Collapse and Recovery of Green Fluorescent Protein Chromophore Emission through Topological Effects

• Published in: Accounts of chemical research Vol. 45; no. 2; pp. 171 - 181
• Main Authors: Tolbert, Laren M, Baldridge, Anthony, Kowalik, Janusz, Solntsev, Kyril M
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 21.02.2012
• Subjects: Chromophores; Collapse; Conjugation; Denaturation; Emission; Fluorescence; Green Fluorescent Proteins - chemistry; Models, Molecular; Molecular Structure; Photochemical; Photochemistry; Protein Conformation; Proteins; Spectrometry, Fluorescence - methods

Housed within the 11-stranded β-barrel of the green fluorescent protein (GFP) is the arylideneimidazolidinone (AMI) chromophore, the component responsible for fluorescence. This class of small-molecule chromophore has drawn significant attention for its remarkable photophysical and photochemical pro...