Multilabeled Pyrene-Functionalized 2‘-Amino-LNA Probes for Nucleic Acid Detection in Homogeneous Fluorescence Assays
Homogeneous fluorescence assays for detection of nucleic acids are widely used in biological sciences. Typically, probes such as molecular beacons that rely on distance-dependent fluorescence quenching are used for such assays. Less attention has been devoted to tethering a single kind of fluorophor...
Saved in:
Published in | Journal of the American Chemical Society Vol. 127; no. 38; pp. 13293 - 13299 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
WASHINGTON
American Chemical Society
28.09.2005
Amer Chemical Soc |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Homogeneous fluorescence assays for detection of nucleic acids are widely used in biological sciences. Typically, probes such as molecular beacons that rely on distance-dependent fluorescence quenching are used for such assays. Less attention has been devoted to tethering a single kind of fluorophores to oligonucleotides and exploiting hybridization-induced modulation of fluorescence intensity for nucleic acid detection. Herein, thermal denaturation experiments and fluorescence properties of oligodeoxyribonucleotides containing one or more 2‘-N-(pyren-1-yl)carbonyl-2‘-amino-LNA monomer(s) X are described. These pyrene-functionalized 2‘-amino-LNAs display large increases in thermal stability against DNA/RNA complements with excellent Watson−Crick mismatch discrimination. Upon duplex formation of appropriately designed 2‘-N-(pyren-1-yl)carbonyl-2‘-amino-LNA probes and complementary DNA/RNA, intensive fluorescence emission with quantum yields between 0.28 and 0.99 are observed. Quantum yields of such magnitudes are unprecedented among pyrene-labeled oligonucleotides. Molecular modeling studies suggest that the dioxabicyclo[2.2.1]heptane skeleton and amide linkage of monomer X fix the orientation of the pyrene moiety in the minor groove of a nucleic acid duplex. Interactions between pyrene and nucleobases, which typically lead to quenching of fluorescence, are thereby reduced. Duplexes between multiple modified probes and DNA/RNA complements exhibit additive increases in fluorescence intensity, while the fluorescence of single stranded probes becomes increasingly quenched. Up to 69-fold increase in fluorescence intensity (measured at λ em = 383 nm) is observed upon hybridization to DNA/RNA. The emission from duplexes of multiple modified probes and DNA/RNA at concentrations down to less than 500 nM can easily be seen by the naked eye using standard illumination intensities. |
---|---|
AbstractList | Homogeneous fluorescence assays for detection of nucleic acids are widely used in biological sciences. Typically, probes such as molecular beacons that rely on distance-dependent fluorescence quenching are used for such assays. Less attention has been devoted to tethering a single kind of fluorophores to oligonucleotides and exploiting hybridization-induced modulation of fluorescence intensity for nucleic acid detection. Herein, thermal denaturation experiments and fluorescence properties of oligodeoxyribonucleotides containing one or more 2'-N-(pyren-1-yl)carbonyl-2'-amino-LNA monomer(s) X are described. These pyrene-functionalized 2'-amino-LNAs display large increases in thermal stability against DNA/RNA complements with excellent Watson-Crick mismatch discrimination. Upon duplex formation of appropriately designed 2'-N-(pyren-1-yl)carbonyl-2'-amino-LNA probes and complementary DNA/RNA, intensive fluorescence emission with quantum yields between 0.28 and 0.99 are observed. Quantum yields of such magnitudes are unprecedented among pyrene-labeled oligonucleotides. Molecular modeling studies suggest that the dioxabicyclo[2.2.1]heptane skeleton and amide linkage of monomer X fix the orientation of the pyrene moiety in the minor groove of a nucleic acid duplex. Interactions between pyrene and nucleobases, which typically lead to quenching of fluorescence, are thereby reduced. Duplexes between multiple modified probes and DNA/RNA complements exhibit additive increases in fluorescence intensity, while the fluorescence of single stranded probes becomes increasingly quenched. Up to 69-fold increase in fluorescence intensity (measured at lambda(em) = 383 nm) is observed upon hybridization to DNA/RNA. The emission from duplexes of multiple modified probes and DNA/RNA at concentrations down to less than 500 nM can easily be seen by the naked eye using standard illumination intensities. Homogeneous fluorescence assays for detection of nucleic acids are widely used in biological sciences. Typically, probes such as molecular beacons that rely on distance-dependent fluorescence quenching are used for such assays. Less attention has been devoted to tethering a single kind of fluorophores to oligonucleotides and exploiting hybridization-induced modulation of fluorescence intensity for nucleic acid detection. Herein, thermal denaturation experiments and fluorescence properties of oligodeoxyribonucleotides containing one or more 2‘-N-(pyren-1-yl)carbonyl-2‘-amino-LNA monomer(s) X are described. These pyrene-functionalized 2‘-amino-LNAs display large increases in thermal stability against DNA/RNA complements with excellent Watson−Crick mismatch discrimination. Upon duplex formation of appropriately designed 2‘-N-(pyren-1-yl)carbonyl-2‘-amino-LNA probes and complementary DNA/RNA, intensive fluorescence emission with quantum yields between 0.28 and 0.99 are observed. Quantum yields of such magnitudes are unprecedented among pyrene-labeled oligonucleotides. Molecular modeling studies suggest that the dioxabicyclo[2.2.1]heptane skeleton and amide linkage of monomer X fix the orientation of the pyrene moiety in the minor groove of a nucleic acid duplex. Interactions between pyrene and nucleobases, which typically lead to quenching of fluorescence, are thereby reduced. Duplexes between multiple modified probes and DNA/RNA complements exhibit additive increases in fluorescence intensity, while the fluorescence of single stranded probes becomes increasingly quenched. Up to 69-fold increase in fluorescence intensity (measured at λ em = 383 nm) is observed upon hybridization to DNA/RNA. The emission from duplexes of multiple modified probes and DNA/RNA at concentrations down to less than 500 nM can easily be seen by the naked eye using standard illumination intensities. Homogeneous fluorescence assays for detection of nucleic acids are widely used in biological sciences. Typically, probes such as molecular beacons that rely on distance-dependent fluorescence quenching are used for such assays, Less attention has been devoted to tethering a single kind of fluorophores to oligonucleotides and exploiting hybridization-induced modulation of fluorescence intensity for nucleic acid detection. Herein, thermal denaturation experiments and fluorescence properties of oligodeoxyribonucleoticles containing one or more 2 '-N-(pyren-1-yl)carbonyl-2 '-amino-LNA monomer(s) X are described. These pyrene-functionalized 2 '-amino-LNAs display large increases in thermal stability against DNA/RNA complements with excellent Watson-Crick mismatch discrimination. Upon duplex formation of appropriately designed 2 '-N-(pyren-1-yl)carbonyl-2 '-amino-LNA probes and complementary DNA/RNA, intensive fluorescence emission with quantum yields between 0.28 and 0.99 are observed. Quantum yields of such magnitudes are unprecedented among pyrene-labeled oligonucleoticles. Molecular modeling studies suggest that the dioxabicyclo[2.2.1]heptane skeleton and amide linkage of monomer X fix the orientation of the pyrene moiety in the minor groove of a nucleic acid duplex. Interactions between pyrene and nucleobases, which typically lead to quenching of fluorescence, are thereby reduced. Duplexes between multiple modified probes and DNA/RNA complements exhibit additive increases in fluorescence intensity, while the fluorescence of single stranded probes becomes increasingly quenched. Up to 69-fold increase in fluorescence intensity (measured lambda(em) = 383 nm) is observed upon hybridization to DNA/RNA. The emission from duplexes of multiple modified probes and DNA/RNA at concentrations down to less than 500 nM can easily be seen by the naked eye using standard illumination intensities. |
Author | Harrit, Niels Sørensen, Mads D Babu, B. Ravindra Hrdlicka, Patrick J Wengel, Jesper |
Author_xml | – sequence: 1 givenname: Patrick J surname: Hrdlicka fullname: Hrdlicka, Patrick J – sequence: 2 givenname: B. Ravindra surname: Babu fullname: Babu, B. Ravindra – sequence: 3 givenname: Mads D surname: Sørensen fullname: Sørensen, Mads D – sequence: 4 givenname: Niels surname: Harrit fullname: Harrit, Niels – sequence: 5 givenname: Jesper surname: Wengel fullname: Wengel, Jesper |
BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17144551$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/16173760$$D View this record in MEDLINE/PubMed |
BookMark | eNqNks2O0zAUhS00iOkUFrwAygYkhAK2kzjJMgRKkcpQiSKxs2znBrk49mAnQFnxGDwfT4I7rdoNC1b-0XeOzj32FbqwzgJCDwl-TjAlL7YCF7SqSnEHzUhBcVoQyi7QDGNM07Ji2SW6CmEbjzmtyD10SRgps5LhGfr2bjKjNkKCgS5Z7zxYSBeTVaN2Vhj9M97SP79-p82grUtX102y9k5CSHrnk-tJGdAqaZTuklcwwq0s0TZZusF9jl5uCsnCTM5DUGAVJE0IYhfuo7u9MAEeHNc5-rh4vWmX6er9m7dts0pFTuiYStkzlsm6VpXoyrpXPe67ThECuZKqVoWgGGeUlhmRoqAl7WusKsVUV8saBGRz9OTge-Pd1wnCyAcdgxgjbqNxVjGCWdTP0dMDqLwLwUPPb7wehN9xgvm-ZH4qObKPjqaTHKA7k8dWI_D4CIighOm9sEqHM1eSPC8KErnqwH0H6fqg9L6hE7Z_vYySEldxx3CrR7Fvt3WTHaP02f9LI50eaB1G-HHChP_CWYxc8M36A8efNmS9pC95ex5AqMC3bvLxK4R_NPEX0iPD6Q |
CODEN | JACSAT |
CitedBy_id | crossref_primary_10_1002_cptc_202000199 crossref_primary_10_1135_cccc2011091 crossref_primary_10_1135_cccc2011096 crossref_primary_10_1016_j_bmc_2011_10_052 crossref_primary_10_1039_C4TB02116A crossref_primary_10_1021_ja801054t crossref_primary_10_1021_ja1057295 crossref_primary_10_1016_j_tetlet_2010_01_081 crossref_primary_10_1016_j_bmcl_2006_03_072 crossref_primary_10_1016_j_bmcl_2009_03_079 crossref_primary_10_1039_b518171b crossref_primary_10_1134_S1068162010060014 crossref_primary_10_1021_jo800551j crossref_primary_10_1016_j_bmc_2022_117148 crossref_primary_10_1021_jo9013657 crossref_primary_10_1038_s41428_022_00623_1 crossref_primary_10_1002_ange_200903194 crossref_primary_10_1039_c2sc20732j crossref_primary_10_1002_cbic_201100689 crossref_primary_10_1021_bc900421r crossref_primary_10_1021_jo061225g crossref_primary_10_1039_b901028a crossref_primary_10_1016_j_jphotochem_2016_07_015 crossref_primary_10_3923_ajbs_2012_257_262 crossref_primary_10_1002_chem_200800514 crossref_primary_10_1021_acs_bioconjchem_6b00529 crossref_primary_10_1039_b922160c crossref_primary_10_1134_S1068162008050105 crossref_primary_10_1016_j_bmcl_2007_10_012 crossref_primary_10_1246_cl_2010_1254 crossref_primary_10_1039_c1ob06325a crossref_primary_10_1002_asia_201402063 crossref_primary_10_1002_anie_201411000 crossref_primary_10_1016_j_bmc_2007_04_058 crossref_primary_10_1039_c2cp40520b crossref_primary_10_1021_bc1005027 crossref_primary_10_1021_ac1004713 crossref_primary_10_1021_ja710342q crossref_primary_10_3923_ajbmb_2013_175_181 crossref_primary_10_1021_cr050266u crossref_primary_10_1039_b802193g crossref_primary_10_1039_C0OB00532K crossref_primary_10_1366_12_06803 crossref_primary_10_1021_ja0634977 crossref_primary_10_1021_acs_analchem_7b03391 crossref_primary_10_1021_bc060279u crossref_primary_10_1002_ange_201304986 crossref_primary_10_1002_ange_201411000 crossref_primary_10_1021_jo801081t crossref_primary_10_1039_c1cs15048k crossref_primary_10_1039_C6PY01075J crossref_primary_10_1135_cccc20060929 crossref_primary_10_1002_cbic_200700144 crossref_primary_10_1039_b705933g crossref_primary_10_1002_chem_201202621 crossref_primary_10_1002_cbdv_200900195 crossref_primary_10_1039_c0cc03026k crossref_primary_10_1080_15257770701542975 crossref_primary_10_1021_bc800202v crossref_primary_10_1021_acs_bioconjchem_9b00746 crossref_primary_10_1021_bc9003926 crossref_primary_10_1039_B706101C crossref_primary_10_1016_j_bmc_2008_08_035 crossref_primary_10_1016_j_bmc_2017_10_008 crossref_primary_10_1016_j_bmcl_2014_08_018 crossref_primary_10_1039_c1cc11021g crossref_primary_10_1021_cm071678h crossref_primary_10_1080_15257770701538841 crossref_primary_10_1039_c3ob40786a crossref_primary_10_1002_cbic_200700408 crossref_primary_10_1002_cptc_201700152 crossref_primary_10_1021_jo500614a crossref_primary_10_3987_COM_18_S_F_46 crossref_primary_10_1002_cphc_201200765 crossref_primary_10_1016_j_tet_2013_10_096 crossref_primary_10_1039_b9pp00016j crossref_primary_10_1039_C5OB00860C crossref_primary_10_1039_c0mb00353k crossref_primary_10_1080_14686996_2016_1182412 crossref_primary_10_1089_adt_2005_3_581 crossref_primary_10_1039_c1cs15014f crossref_primary_10_1021_cr100351g crossref_primary_10_1002_cbic_200700533 crossref_primary_10_1002_chem_201501955 crossref_primary_10_1021_ja071076z crossref_primary_10_1002_cbic_201402105 crossref_primary_10_1016_j_bmcl_2010_10_089 crossref_primary_10_1016_j_tet_2013_03_054 crossref_primary_10_1246_bcsj_20140372 crossref_primary_10_1016_j_aca_2013_08_012 crossref_primary_10_1080_15257770701539153 crossref_primary_10_1002_anie_200903194 crossref_primary_10_1002_anie_201304986 crossref_primary_10_1021_bc100090y crossref_primary_10_1021_acs_analchem_6b00227 crossref_primary_10_1039_c3sc50726b crossref_primary_10_1021_jo302036h crossref_primary_10_1021_jo702747w crossref_primary_10_1089_adt_2008_9986 crossref_primary_10_1021_ar500014g crossref_primary_10_1002_chem_201002109 crossref_primary_10_1002_tcr_201402040 crossref_primary_10_1039_c0cc01133a crossref_primary_10_1002_chem_201204183 crossref_primary_10_1021_jo060331f crossref_primary_10_1196_annals_1430_001 crossref_primary_10_1021_ja0581806 crossref_primary_10_1016_j_bmcl_2012_03_073 crossref_primary_10_1021_jo802037v crossref_primary_10_1039_D2OB01809H crossref_primary_10_1016_j_tetlet_2008_09_165 crossref_primary_10_1016_j_tet_2006_12_097 crossref_primary_10_1016_j_polymer_2012_12_080 crossref_primary_10_1016_j_tet_2013_12_013 crossref_primary_10_1016_j_bmc_2009_05_074 crossref_primary_10_1016_j_bmcl_2022_128838 crossref_primary_10_1039_b515108m crossref_primary_10_1021_bc700280h crossref_primary_10_1016_j_carres_2023_108857 crossref_primary_10_1002_chem_200800380 crossref_primary_10_1002_chem_200901607 crossref_primary_10_1021_ja805502k crossref_primary_10_1002_chem_200801077 |
Cites_doi | 10.1002/cbic.200400161 10.1039/b307026c 10.1039/b111434b 10.1002/cbic.200400260 10.1039/b417101b 10.1021/jo049824f 10.1021/bc025530u 10.1073/pnas.2233244100 10.1016/S0968-0896(03)00227-X 10.1002/chem.200304941 10.1021/ja045207x 10.1002/cbic.200300662 10.1016/S0167-7799(02)00038-0 10.1021/ja039625y 10.1039/b313986g 10.1006/mcpr.2001.0384 10.1016/j.tibtech.2005.03.007 10.1002/cbic.200400325 10.1126/science.1455230 10.1021/cr00018a001 10.1016/S0040-4039(98)01084-3 10.1002/1521-3773(20021004)41:19<3648::AID-ANIE3648>3.0.CO;2-Y 10.1038/359859a0 10.1562/0031-8655(2002)075<0201:FPOOCT>2.0.CO;2 10.1093/nar/27.11.2387 10.1016/S0002-9440(10)63627-9 10.1023/A:1022551516070 10.1021/ja002294u 10.1021/bc049976h 10.1039/B313986G 10.1093/nar/24.24.4992 10.1021/j100048a024 10.1021/jp000827f 10.1093/nar/25.14.2923 10.1021/ja00449a004 10.1038/11751 10.1006/abio.2000.4957 10.1006/abio.2000.4534 10.1002/1521-3773(20010316)40:6<1104::AID-ANIE11040>3.0.CO;2-2 10.1093/nar/25.12.2516 10.1016/S1011-1344(00)00127-5 10.1021/j100550a010 10.1021/ar980051p 10.1002/jcc.540110405 10.1093/clinchem/43.5.752 10.1016/S0040-4020(98)00094-5 10.1021/ja00315a052 |
ContentType | Journal Article |
Copyright | Copyright © 2005 American Chemical Society 2005 INIST-CNRS |
Copyright_xml | – notice: Copyright © 2005 American Chemical Society – notice: 2005 INIST-CNRS |
DBID | BSCLL 1KM BLEPL DTL FZXEZ IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
DOI | 10.1021/ja052887a |
DatabaseName | Istex Index Chemicus Web of Science Core Collection Science Citation Index Expanded Web of Science - Science Citation Index Expanded - 2005 Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic |
DatabaseTitle | Web of Science MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
DatabaseTitleList | MEDLINE MEDLINE - Academic Web of Science |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 1KM name: Index Chemicus url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/woscc/search-with-editions?editions=WOS.IC sourceTypes: Enrichment Source Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Chemistry |
EISSN | 1520-5126 |
EndPage | 13299 |
ExternalDocumentID | 10_1021_ja052887a 16173760 17144551 000232170800060 ark_67375_TPS_0XT1PH2B_C i29179250 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | - .K2 02 186 4.4 53G 55A 5GY 5RE 5VS 7~N 85S AABXI ABDEX ABFLS ABMVS ABPPZ ABPTK ABUCX ABUFD ACGFS ACJ ACNCT ACS AEESW AENEX AETEA AFEFF AFFDN AFFNX AFMIJ AIDAL ALMA_UNASSIGNED_HOLDINGS ANTXH AQSVZ BAANH BKOMP CS3 DU5 DZ EBS ED ED~ EJD ET F20 F5P GJ GNL IH9 IHE JG JG~ K2 K78 LG6 NHB OHT P2P ROL RXW TAE TAF TN5 UHB UI2 UKR UNC UPT UQL VF5 VG9 VQA W1F WH7 X XFK YZZ ZCG ZE2 ZHY --- -DZ -ET -~X .DC .GJ 6TJ AAYOK ABFRP ABJNI ABQRX ACBEA ACGFO ADHLV AGHSJ AGXLV AHGAQ BSCLL GGK IH2 XOL XSW YQT ZCA ~02 1KM AAHBH BLEPL CUPRZ DTL GROUPED_WOS_SCIENCE_CITATION_INDEX_EXPANDED .HR 1WB 3EH 3O- 41~ AAUPJ AAYJJ ABHMW ABTAH ABWLT ACBNA ACKIV AFDAS AGJRR AI. D0S IQODW MVM P-O RNS UBC UBX VH1 X7L YXA YXE YYP ZGI ZY4 CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
ID | FETCH-LOGICAL-a412t-bbf663b99c8ad79fcf0fddc11e4cbc9c5a200322731ba5272f90c8c6cd9b9eae3 |
IEDL.DBID | ACS |
ISICitedReferencesCount | 118 |
ISICitedReferencesURI | https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestApp=WOS&DestLinkType=CitingArticles&UT=000232170800060 |
ISSN | 0002-7863 |
IngestDate | Fri Aug 16 11:19:05 EDT 2024 Fri Aug 23 02:03:40 EDT 2024 Thu May 23 23:11:22 EDT 2024 Sun Oct 29 17:08:03 EDT 2023 Wed Sep 18 03:35:16 EDT 2024 Wed Sep 18 05:51:08 EDT 2024 Wed Jan 17 04:52:10 EST 2024 Thu Aug 27 13:43:01 EDT 2020 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 38 |
Keywords | POLYMERASE-CHAIN-REACTION ELECTRON-TRANSFER THIAZOLE ORANGE DNA PROBES FLUOROGENIC PROBES LABELED OLIGONUCLEOTIDES MOLECULAR BEACONS QUENCHED PROBES LIGHT-UP PROBES MONOMER FLUORESCENCE Biochemical analysis Functionalization Fluorescent labelling Pyrene derivatives Thermal denaturation Qualitative analysis Oligonucleotide Oligodeoxyribonucleotide Quantum yield Nucleic acid Fluorescence spectrometry |
Language | English |
License | CC BY 4.0 |
LinkModel | DirectLink |
LogoURL | https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg |
MergedId | FETCHMERGED-LOGICAL-a412t-bbf663b99c8ad79fcf0fddc11e4cbc9c5a200322731ba5272f90c8c6cd9b9eae3 |
Notes | istex:ECFDB785F0D83547E12B869C0E69CB552F264E90 ark:/67375/TPS-0XT1PH2B-C A research center funded by the Danish National Research Foundation for studies on nucleic acid chemical biology. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ORCID | 0000-0001-9835-1009 0000-0002-8113-0450 |
PMID | 16173760 |
PQID | 68610627 |
PQPubID | 23479 |
PageCount | 7 |
ParticipantIDs | pubmed_primary_16173760 pascalfrancis_primary_17144551 acs_journals_10_1021_ja052887a proquest_miscellaneous_68610627 crossref_primary_10_1021_ja052887a webofscience_primary_000232170800060CitationCount webofscience_primary_000232170800060 istex_primary_ark_67375_TPS_0XT1PH2B_C |
ProviderPackageCode | JG~ 55A AABXI GNL VF5 7~N ACJ VG9 W1F ANTXH ACS AEESW AFEFF .K2 ABMVS ABUCX IH9 BAANH AQSVZ ED~ UI2 |
PublicationCentury | 2000 |
PublicationDate | 2005-09-28 |
PublicationDateYYYYMMDD | 2005-09-28 |
PublicationDate_xml | – month: 09 year: 2005 text: 2005-09-28 day: 28 |
PublicationDecade | 2000 |
PublicationPlace | WASHINGTON |
PublicationPlace_xml | – name: WASHINGTON – name: Washington, DC – name: United States |
PublicationTitle | Journal of the American Chemical Society |
PublicationTitleAbbrev | J AM CHEM SOC |
PublicationTitleAlternate | J. Am. Chem. Soc |
PublicationYear | 2005 |
Publisher | American Chemical Society Amer Chemical Soc |
Publisher_xml | – name: American Chemical Society – name: Amer Chemical Soc |
References | Misra, A (WOS:000221563300024) 2004; 15 Johansson, MK (WOS:000184679700001) 2003; 9 Yamana, K (WOS:000167548900030) 2001; 40 Silverman, SK (WOS:000083417800013) 1999; 38 BEVILACQUA, PC (WOS:A1992JY95800038) 1992; 258 Kohler, O (WOS:000226328900011) 2005; 6 NAZA (WOS:000232170800060.28) 1997; 25 Wengel, J (WOS:000079922500005) 1999; 32 Silverman, AP (WOS:000229466000004) 2005; 23 WINNIK, FM (WOS:A1993KW29700002) 1993; 93 Wilhelm, J (WOS:000186591300001) 2003; 4 Yamana, K (WOS:000179517700014) 2002; 13 Marks, AHR (WOS:000230043100005) 2005; 23 Whitcombe, D (WOS:000081751400032) 1999; 17 GLAZER, AN (WOS:A1992JV77700073) 1992; 359 MOHAMADI RD (WOS:000232170800060.24) 1990; 11 French, DJ (WOS:000174026800006) 2001; 15 Morrison, LE (WOS:000082923400005) 1999; 9 Ishiguro, T (WOS:A1996WA46200021) 1996; 24 BABU BR (WOS:000232170800060.2) Mahara, A (WOS:000178609900025) 2002; 41 Gelmini, S (WOS:A1997WY18400009) 1997; 43 SEITZ O (WOS:000232170800060.36) 2004 Singh, SK (WOS:000072236700005) 1998 Bratu, DP (WOS:000186573700037) 2003; 100 Yamana, K (WOS:000080678100021) 1999; 27 Okamoto, A (WOS:000220849900030) 2004; 126 Nakatani, K (WOS:000225672400001) 2004; 5 MANOHARAN, M (WOS:A1995TH23600024) 1995; 99 Svanvik, N (WOS:000166843900003) 2001; 123 WEINER, SJ (WOS:A1984SC63100051) 1984; 106 Petersen, M (WOS:000181154900007) 2003; 21 Dohno, C (WOS:000229730000020) 2005; 6 Woods, KK (WOS:000225349800006) 2004; 126 Korshun, VA (WOS:000175001100012) 2002 Obika, S (WOS:000074562000031) 1998; 39 KALYANASUNDARAM, K (WOS:A1977CZ99900004) 1977; 99 HIGUCHI, R (WOS:A1993LU95700021) 1993; 11 Sorensen, MD (WOS:000184684500013) 2003 Koshkin, AA (WOS:000072539000023) 1998; 54 Netzel, TL (WOS:A1995TL76100020) 1995; 99 Vaughn, CP (WOS:000183829900004) 2003; 163 Hrdlicka, PJ (WOS:000222899400022) 2004 Petty, JT (WOS:000088522400036) 2000; 104 MORRIS, JV (WOS:A1976BP02400010) 1976; 80 Privat, E (WOS:000174741400002) 2002; 75 Babu, BR (WOS:000227893300011) 2005 Singh, SK (WOS:000077834800076) 1998; 63 Crockett, AO (WOS:000167283300011) 2001; 290 Tyagi, S (WOS:A1996UL38000022) 1996; 14 Talavera, EM (WOS:000168001300002) 2000; 59 Mahara, A (WOS:000183590100013) 2003; 11 Randolph, JB (WOS:A1997XL84100034) 1997; 25 Fujimoto, K (WOS:000221341100004) 2004; 69 Wengel, J (WOS:000189146100001) 2004; 2 Svanvik, N (WOS:000087282700005) 2000; 281 Weiner S. J. (ja052887ab00059/ja052887ab00059_1) 1984; 106 Randolph J. B. (ja052887ab00026/ja052887ab00026_1) 1997; 25 Petersen M. (ja052887ab00064/ja052887ab00064_1) 2003; 21 Yamana K. (ja052887ab00029/ja052887ab00029_1) 2001; 40 Okamoto A. (ja052887ab00034/ja052887ab00034_1) 2004; 126 Seitz O. (ja052887ab00007/ja052887ab00007_1) 2004 Winnik F. M. (ja052887ab00062/ja052887ab00062_1) 1993; 93 Dohno C. (ja052887ab00035/ja052887ab00035_1) 2005; 6 Woods K. K. (ja052887ab00049/ja052887ab00049_1) 2004; 126 Obika S. (ja052887ab00038/ja052887ab00038_1) 1998; 39 Netzel T. L. (ja052887ab00051/ja052887ab00051_1) 1995; 99 Morrison L. E. (ja052887ab00008/ja052887ab00008_1) 1999; 9 Mahara A. (ja052887ab00006/ja052887ab00006_1) 2003; 11 Silverman S. K. (ja052887ab00005/ja052887ab00005_1) 1999; 38 Wengel J. (ja052887ab00046/ja052887ab00046_1) 2004; 2 Privat E. (ja052887ab00013/ja052887ab00013_1) 2002; 75 Kalyanasundaram K. (ja052887ab00057/ja052887ab00057_1) 1977; 99 Svanvik N. (ja052887ab00014/ja052887ab00014_1) 2000; 281 Mohamadi R. D. (ja052887ab00060/ja052887ab00060_1) 1990; 11 Manoharan M. (ja052887ab00054/ja052887ab00054_1) 1995; 99 Babu B. R. (ja052887ab00045/ja052887ab00045_1) 2005; 1705 Gelmini S. (ja052887ab00020/ja052887ab00020_1) 1997; 43 Singh S. K. (ja052887ab00042/ja052887ab00042_1) 1998; 63 Higuchi R. (ja052887ab00010/ja052887ab00010_1) 1993; 11 French D. J. (ja052887ab00024/ja052887ab00024_1) 2001; 15 Bevilacqua P. C. (ja052887ab00004/ja052887ab00004_1) 1992; 258 Petty J. T. (ja052887ab00011/ja052887ab00011_1) 2000; 104 Singh S. K. (ja052887ab00037/ja052887ab00037_1) 1998; 455 Wengel J. (ja052887ab00039/ja052887ab00039_1) 1999; 32 Whitcombe D. (ja052887ab00021/ja052887ab00021_1) 1999; 17 Sørensen M. D. (ja052887ab00043/ja052887ab00043_1) 2003; 2130 Talavera E. M. (ja052887ab00022/ja052887ab00022_1) 2000; 59 Bratu D. P. (ja052887ab00002/ja052887ab00002_1) 2003; 100 Nakatani K. (ja052887ab00003/ja052887ab00003_1) 2004; 5 Fujimoto K. (ja052887ab00033/ja052887ab00033_1) 2004; 69 Tyagi S. (ja052887ab00018/ja052887ab00018_1) 1996; 14 Köhler O. (ja052887ab00015/ja052887ab00015_1) 2005; 6 Johansson M. K. (ja052887ab00017/ja052887ab00017_1) 2003; 9 ja052887ab00040/ja052887ab00040_1 Svanvik N. (ja052887ab00016/ja052887ab00016_1) 2001; 123 Morris J. V. (ja052887ab00052/ja052887ab00052_1) 1976; 80 Crockett A. O. (ja052887ab00023/ja052887ab00023_1) 2001; 290 Wilhelm J. (ja052887ab00001/ja052887ab00001_1) 2003; 4 Glazer A. N. (ja052887ab00009/ja052887ab00009_1) 1992; 359 Yamana K. (ja052887ab00030/ja052887ab00030_1) 2002; 13 Mahara A. (ja052887ab00032/ja052887ab00032_1) 2002; 41 Yamana K. (ja052887ab00028/ja052887ab00028_1) 1999; 27 Nazarenko I. A. (ja052887ab00019/ja052887ab00019_1) 1997; 25 Misra A. (ja052887ab00027/ja052887ab00027_1) 2004; 15 Korshun V. A. (ja052887ab00031/ja052887ab00031_1) 2002; 1092 Marks A. H. R. (ja052887ab00058/ja052887ab00058_1) 2005; 23 For (ja052887ab00063/ja052887ab00063_1) 2005; 23 Ishiguro T. (ja052887ab00012/ja052887ab00012_1) 1996; 24 Vaughn C. P. (ja052887ab00025/ja052887ab00025_1) 2003; 163 Hrdlicka P. J. (ja052887ab00044/ja052887ab00044_1) 2004; 1478 |
References_xml | – volume: 5 start-page: 1623 year: 2004 ident: WOS:000225672400001 article-title: Chemistry challenges in SNP typing publication-title: CHEMBIOCHEM doi: 10.1002/cbic.200400161 contributor: fullname: Nakatani, K – start-page: 2130 year: 2003 ident: WOS:000184684500013 article-title: Functionalized LNA (locked nucleic acid): high-affinity hybridization of oligonucleotides containing N-acylated and N-alkylated 2 '-amino-LNA monomers publication-title: CHEMICAL COMMUNICATIONS doi: 10.1039/b307026c contributor: fullname: Sorensen, MD – volume: 9 start-page: 187 year: 1999 ident: WOS:000082923400005 article-title: Homogeneous detection of specific DNA sequences by fluorescence quenching and energy transfer publication-title: JOURNAL OF FLUORESCENCE contributor: fullname: Morrison, LE – volume: 106 start-page: 765 year: 1984 ident: WOS:A1984SC63100051 article-title: A NEW FORCE-FIELD FOR MOLECULAR MECHANICAL SIMULATION OF NUCLEIC-ACIDS AND PROTEINS publication-title: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY contributor: fullname: WEINER, SJ – start-page: 1092 year: 2002 ident: WOS:000175001100012 article-title: Novel uridin-2 '-yl carbamates: synthesis, incorporation into oligodeoxyribonucleotides, and remarkable fluorescence properties of 2 '-pyren-1-ylmethylcarbamate publication-title: JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1 doi: 10.1039/b111434b contributor: fullname: Korshun, VA – volume: 6 start-page: 69 year: 2005 ident: WOS:000226328900011 article-title: Forced intercalation probes (FIT probes): Thiazole orange as a fluorescent base in peptide nucleic acids for homogeneous single-nucleotide-polymorphism detection publication-title: CHEMBIOCHEM doi: 10.1002/cbic.200400260 contributor: fullname: Kohler, O – start-page: 1705 year: 2005 ident: WOS:000227893300011 article-title: Optimized DNA targeting using N,N-bis(2-pyridylmethyl)-beta-alanyl 2 '-amino-LNA publication-title: CHEMICAL COMMUNICATIONS doi: 10.1039/b417101b contributor: fullname: Babu, BR – volume: 14 start-page: 303 year: 1996 ident: WOS:A1996UL38000022 article-title: Molecular beacons: Probes that fluoresce upon hybridization publication-title: NATURE BIOTECHNOLOGY contributor: fullname: Tyagi, S – volume: 41 start-page: 3648 year: 2002 ident: WOS:000178609900025 article-title: Bispyrene-conjugated 2 '-O-methyloligonucleotide as a highly specific RNA-recognition probe publication-title: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION contributor: fullname: Mahara, A – start-page: 311 year: 2004 ident: WOS:000232170800060.36 publication-title: HIGHLIGHTS BIOORGANI contributor: fullname: SEITZ O – volume: 27 start-page: 2387 year: 1999 ident: WOS:000080678100021 article-title: 2 '-Pyrene modified oligonucleotide provides a highly sensitive fluorescent probe of RNA publication-title: NUCLEIC ACIDS RESEARCH contributor: fullname: Yamana, K – ident: WOS:000232170800060.2 publication-title: UNPUB contributor: fullname: BABU BR – volume: 69 start-page: 3271 year: 2004 ident: WOS:000221341100004 article-title: Unambiguous detection of target DNAs by excimer-monomer switching molecular beacons publication-title: JOURNAL OF ORGANIC CHEMISTRY doi: 10.1021/jo049824f contributor: fullname: Fujimoto, K – volume: 281 start-page: 26 year: 2000 ident: WOS:000087282700005 article-title: Light-up probes: Thiazole orange-conjugated peptide nucleic acid for detection of target nucleic acid in homogeneous solution publication-title: ANALYTICAL BIOCHEMISTRY contributor: fullname: Svanvik, N – volume: 39 start-page: 5401 year: 1998 ident: WOS:000074562000031 article-title: Stability and structural features of the duplexes containing nucleoside analogues with a fixed N-type conformation, 2 '-O,4 '-C-methyleneribonucleosides publication-title: TETRAHEDRON LETTERS contributor: fullname: Obika, S – volume: 99 start-page: 2039 year: 1977 ident: WOS:A1977CZ99900004 article-title: ENVIRONMENTAL EFFECTS ON VIBRONIC BAND INTENSITIES IN PYRENE MONOMER FLUORESCENCE AND THEIR APPLICATION IN STUDIES OF MICELLAR SYSTEMS publication-title: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY contributor: fullname: KALYANASUNDARAM, K – volume: 290 start-page: 89 year: 2001 ident: WOS:000167283300011 article-title: Fluorescein-labeled oligonucleotides for real-time PCR: Using the inherent quenching of deoxyguanosine nucleotides publication-title: ANALYTICAL BIOCHEMISTRY contributor: fullname: Crockett, AO – start-page: 455 year: 1998 ident: WOS:000072236700005 article-title: LNA (locked nucleic acids): synthesis and high-affinity nucleic acid recognition publication-title: CHEMICAL COMMUNICATIONS contributor: fullname: Singh, SK – volume: 40 start-page: 1104 year: 2001 ident: WOS:000167548900030 article-title: Fluorescence detection of specific RNA sequences using 2'-pyrene-modified oligoribonucleotides publication-title: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION contributor: fullname: Yamana, K – volume: 13 start-page: 1266 year: 2002 ident: WOS:000179517700014 article-title: Bis-pyrene-labeled oligonucleotides: Sequence specificity of excimer and monomer fluorescence changes upon hybridization with DNA publication-title: BIOCONJUGATE CHEMISTRY doi: 10.1021/bc025530u contributor: fullname: Yamana, K – volume: 99 start-page: 17461 year: 1995 ident: WOS:A1995TH23600024 article-title: BASE-SEQUENCE DEPENDENCE OF EMISSION LIFETIMES FOR DNA OLIGOMERS AND DUPLEXES COVALENTLY LABELED WITH PYRENE - RELATIVE ELECTRON-TRANSFER QUENCHING EFFICIENCIES OF A-NUCLEOSIDE, G-NUCLEOSIDE, C-NUCLEOSIDE, AND T-NUCLEOSIDE TOWARD PYRENE publication-title: JOURNAL OF PHYSICAL CHEMISTRY contributor: fullname: MANOHARAN, M – volume: 100 start-page: 13308 year: 2003 ident: WOS:000186573700037 article-title: Visualizing the distribution and transport of mRNAs in living cells publication-title: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA doi: 10.1073/pnas.2233244100 contributor: fullname: Bratu, DP – volume: 11 start-page: 1026 year: 1993 ident: WOS:A1993LU95700021 article-title: KINETIC PCR ANALYSIS - REAL-TIME MONITORING OF DNA AMPLIFICATION REACTIONS publication-title: BIO-TECHNOLOGY contributor: fullname: HIGUCHI, R – volume: 59 start-page: 9 year: 2000 ident: WOS:000168001300002 article-title: Fluorescence-labelled DNA probes to detect complementary sequences in homogeneous media publication-title: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY contributor: fullname: Talavera, EM – volume: 63 start-page: 10035 year: 1998 ident: WOS:000077834800076 article-title: Synthesis of 2 '-amino-LNA: A novel conformationally restricted high-affinity oligonucleotide analogue with a handle publication-title: JOURNAL OF ORGANIC CHEMISTRY contributor: fullname: Singh, SK – start-page: 1478 year: 2004 ident: WOS:000222899400022 article-title: Interstrand communication between 2 '-N-(pyren-1-yl)methyl-2 '-amino-LNA monomers in nucleic acid duplexes: directional control and signalling of full complementarity publication-title: CHEMICAL COMMUNICATIONS contributor: fullname: Hrdlicka, PJ – volume: 23 start-page: 49 year: 2005 ident: WOS:000230043100005 article-title: Molecular basis of action of HyBeacon (TM) fluorogenic probes: A spectroscopic and molecular dynamics study publication-title: JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS contributor: fullname: Marks, AHR – volume: 17 start-page: 804 year: 1999 ident: WOS:000081751400032 article-title: Detection of PCR products using self-probing amplicons and fluorescence publication-title: NATURE BIOTECHNOLOGY contributor: fullname: Whitcombe, D – volume: 11 start-page: 2783 year: 2003 ident: WOS:000183590100013 article-title: Detection of acceptor sites for antisense oligonucleotides on native folded RNA by fluorescence spectroscopy publication-title: BIOORGANIC & MEDICINAL CHEMISTRY doi: 10.1016/S0968-0896(03)00227-X contributor: fullname: Mahara, A – volume: 32 start-page: 301 year: 1999 ident: WOS:000079922500005 article-title: Synthesis of 3 '-C- and 4 '-C-branched oligodeoxynucleotides and the development of locked nucleic acid (LNA) publication-title: ACCOUNTS OF CHEMICAL RESEARCH contributor: fullname: Wengel, J – volume: 9 start-page: 3466 year: 2003 ident: WOS:000184679700001 article-title: Intramolecular dimers: A new design strategy for fluorescence-quenched probes publication-title: CHEMISTRY-A EUROPEAN JOURNAL doi: 10.1002/chem.200304941 contributor: fullname: Johansson, MK – volume: 43 start-page: 752 year: 1997 ident: WOS:A1997WY18400009 article-title: Quantitative polymerase chain reaction-based homogeneous assay with fluorogenic probes to measure c-erbB-2 oncogene amplification publication-title: CLINICAL CHEMISTRY contributor: fullname: Gelmini, S – volume: 126 start-page: 15330 year: 2004 ident: WOS:000225349800006 article-title: High-resolution structure of an extended A-tract: [d(CGCAAATTTGCG)](2) publication-title: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY doi: 10.1021/ja045207x contributor: fullname: Woods, KK – volume: 54 start-page: 3607 year: 1998 ident: WOS:000072539000023 article-title: LNA (Locked Nucleic Acids): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recognition publication-title: TETRAHEDRON contributor: fullname: Koshkin, AA – volume: 4 start-page: 1120 year: 2003 ident: WOS:000186591300001 article-title: Real-time polymerase chain reaction publication-title: CHEMBIOCHEM doi: 10.1002/cbic.200300662 contributor: fullname: Wilhelm, J – volume: 104 start-page: 7221 year: 2000 ident: WOS:000088522400036 article-title: Thermodynamic characterization of the association of cyanine dyes with DNA publication-title: JOURNAL OF PHYSICAL CHEMISTRY B contributor: fullname: Petty, JT – volume: 75 start-page: 201 year: 2002 ident: WOS:000174741400002 article-title: Fluorescent properties of oligonucleotide-conjugated thiazole orange probes publication-title: PHOTOCHEMISTRY AND PHOTOBIOLOGY contributor: fullname: Privat, E – volume: 25 start-page: 2516 year: 1997 ident: WOS:000232170800060.28 publication-title: NUCL ACIDS RES contributor: fullname: NAZA – volume: 21 start-page: 74 year: 2003 ident: WOS:000181154900007 article-title: LNA: a versatile tool for therapeutics and genomics publication-title: TRENDS IN BIOTECHNOLOGY doi: 10.1016/S0167-7799(02)00038-0 contributor: fullname: Petersen, M – volume: 93 start-page: 587 year: 1993 ident: WOS:A1993KW29700002 article-title: PHOTOPHYSICS OF PREASSOCIATED PYRENES IN AQUEOUS POLYMER-SOLUTIONS AND IN OTHER ORGANIZED MEDIA publication-title: CHEMICAL REVIEWS contributor: fullname: WINNIK, FM – volume: 258 start-page: 1355 year: 1992 ident: WOS:A1992JY95800038 article-title: DYNAMICS OF RIBOZYME BINDING OF SUBSTRATE REVEALED BY FLUORESCENCE-DETECTED STOPPED-FLOW METHODS publication-title: SCIENCE contributor: fullname: BEVILACQUA, PC – volume: 99 start-page: 17948 year: 1995 ident: WOS:A1995TL76100020 article-title: Direct observation of photoinduced electron transfer in pyrene-labeled dU nucleosides and evidence for protonated 2'-deoxyuridine anion, dU(H)(center dot), as a primary electron transfer product publication-title: JOURNAL OF PHYSICAL CHEMISTRY contributor: fullname: Netzel, TL – volume: 11 start-page: 440 year: 1990 ident: WOS:000232170800060.24 publication-title: J COMPUT CHEM contributor: fullname: MOHAMADI RD – volume: 25 start-page: 2923 year: 1997 ident: WOS:A1997XL84100034 article-title: Stability, specificity and fluorescence brightness of multiply-labeled fluorescent DNA probes publication-title: NUCLEIC ACIDS RESEARCH contributor: fullname: Randolph, JB – volume: 24 start-page: 4992 year: 1996 ident: WOS:A1996WA46200021 article-title: Fluorescence detection of specific sequence of nucleic acids by oxazole yellow-linked oligonucleotides. Homogeneous quantitative monitoring of in vitro transcription publication-title: NUCLEIC ACIDS RESEARCH contributor: fullname: Ishiguro, T – volume: 15 start-page: 638 year: 2004 ident: WOS:000221563300024 article-title: Synthesis and fluorescence studies of multiple labeled oligonucleotides containing dansyl fluorophore covalently attached at 2'-terminus of cytidine via carbamate linkage publication-title: BIOCONJUGATE CHEMISTRY contributor: fullname: Misra, A – volume: 126 start-page: 4820 year: 2004 ident: WOS:000220849900030 article-title: Pyrene-labeled base-discriminating fluorescent DNA probes for homogeneous SNP typing publication-title: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY doi: 10.1021/ja039625y contributor: fullname: Okamoto, A – volume: 123 start-page: 803 year: 2001 ident: WOS:000166843900003 article-title: Free-probe fluorescence of light-up probes publication-title: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY contributor: fullname: Svanvik, N – volume: 80 start-page: 969 year: 1976 ident: WOS:A1976BP02400010 article-title: FLUORESCENCE QUANTUM YIELD DETERMINATIONS - 9,10-DIPHENYLANTHRACENE AS A REFERENCE-STANDARD IN DIFFERENT SOLVENTS publication-title: JOURNAL OF PHYSICAL CHEMISTRY contributor: fullname: MORRIS, JV – volume: 359 start-page: 859 year: 1992 ident: WOS:A1992JV77700073 article-title: STABLE DYE-DNA INTERCALATION COMPLEXES AS REAGENTS FOR HIGH-SENSITIVITY FLUORESCENCE DETECTION publication-title: NATURE contributor: fullname: GLAZER, AN – volume: 2 start-page: 277 year: 2004 ident: WOS:000189146100001 article-title: Nucleic acid nanotechnology - towards Angstrom-scale engineering publication-title: ORGANIC & BIOMOLECULAR CHEMISTRY doi: 10.1039/b313986g contributor: fullname: Wengel, J – volume: 38 start-page: 14224 year: 1999 ident: WOS:000083417800013 article-title: RNA tertiary folding monitored by fluorescence of covalently attached pyrene publication-title: BIOCHEMISTRY contributor: fullname: Silverman, SK – volume: 15 start-page: 363 year: 2001 ident: WOS:000174026800006 article-title: HyBeacon (TM) probes: a new tool for DNA sequence detection and allele discrimination publication-title: MOLECULAR AND CELLULAR PROBES doi: 10.1006/mcpr.2001.0384 contributor: fullname: French, DJ – volume: 163 start-page: 29 year: 2003 ident: WOS:000183829900004 article-title: Hybridization induced dequenching of fluorescein-labeled oligonucleotides - A novel strategy for PCR detection and genotyping publication-title: AMERICAN JOURNAL OF PATHOLOGY contributor: fullname: Vaughn, CP – volume: 23 start-page: 225 year: 2005 ident: WOS:000229466000004 article-title: Quenched probes for highly specific detection of cellular RNAs publication-title: TRENDS IN BIOTECHNOLOGY doi: 10.1016/j.tibtech.2005.03.007 contributor: fullname: Silverman, AP – volume: 6 start-page: 1075 year: 2005 ident: WOS:000229730000020 article-title: Discrimination of single-nucleotide alterations by G-specific fluorescence quenching publication-title: CHEMBIOCHEM doi: 10.1002/cbic.200400325 contributor: fullname: Dohno, C – volume: 2130 start-page: 2131 year: 2003 ident: ja052887ab00043/ja052887ab00043_1 publication-title: Chem. Commun. contributor: fullname: Sørensen M. D. – volume: 258 start-page: 1358 year: 1992 ident: ja052887ab00004/ja052887ab00004_1 publication-title: Science doi: 10.1126/science.1455230 contributor: fullname: Bevilacqua P. C. – volume: 93 start-page: 614 year: 1993 ident: ja052887ab00062/ja052887ab00062_1 publication-title: Chem. Rev. doi: 10.1021/cr00018a001 contributor: fullname: Winnik F. M. – volume: 39 start-page: 5404 year: 1998 ident: ja052887ab00038/ja052887ab00038_1 publication-title: Tetrahedron Lett. doi: 10.1016/S0040-4039(98)01084-3 contributor: fullname: Obika S. – volume: 11 start-page: 1030 year: 1993 ident: ja052887ab00010/ja052887ab00010_1 publication-title: BioTechnology contributor: fullname: Higuchi R. – volume: 41 start-page: 3650 year: 2002 ident: ja052887ab00032/ja052887ab00032_1 publication-title: Angew. Chem. Int. Ed. doi: 10.1002/1521-3773(20021004)41:19<3648::AID-ANIE3648>3.0.CO;2-Y contributor: fullname: Mahara A. – volume: 23 start-page: 62 year: 2005 ident: ja052887ab00058/ja052887ab00058_1 publication-title: J. Biolmol. Struct. Dyn. contributor: fullname: Marks A. H. R. – volume: 359 start-page: 860 year: 1992 ident: ja052887ab00009/ja052887ab00009_1 publication-title: Nature doi: 10.1038/359859a0 contributor: fullname: Glazer A. N. – volume: 6 start-page: 1081 year: 2005 ident: ja052887ab00035/ja052887ab00035_1 publication-title: ChemBioChem doi: 10.1002/cbic.200400325 contributor: fullname: Dohno C. – volume: 75 start-page: 210 year: 2002 ident: ja052887ab00013/ja052887ab00013_1 publication-title: Photochem. Photobiol. doi: 10.1562/0031-8655(2002)075<0201:FPOOCT>2.0.CO;2 contributor: fullname: Privat E. – volume: 27 start-page: 2392 year: 1999 ident: ja052887ab00028/ja052887ab00028_1 publication-title: Nucleic Acids Res. doi: 10.1093/nar/27.11.2387 contributor: fullname: Yamana K. – volume: 163 start-page: 35 year: 2003 ident: ja052887ab00025/ja052887ab00025_1 publication-title: Am. J. Pathol. doi: 10.1016/S0002-9440(10)63627-9 contributor: fullname: Vaughn C. P. – volume: 23 start-page: 230 year: 2005 ident: ja052887ab00063/ja052887ab00063_1 publication-title: Trends Biotechnol. contributor: fullname: For – volume: 13 start-page: 1273 year: 2002 ident: ja052887ab00030/ja052887ab00030_1 publication-title: Bioconjuate Chem. doi: 10.1021/bc025530u contributor: fullname: Yamana K. – volume: 14 start-page: 308 year: 1996 ident: ja052887ab00018/ja052887ab00018_1 publication-title: Nat. Biotechnol. contributor: fullname: Tyagi S. – volume: 9 start-page: 196 year: 1999 ident: ja052887ab00008/ja052887ab00008_1 publication-title: J. Fluoresc. doi: 10.1023/A:1022551516070 contributor: fullname: Morrison L. E. – volume: 63 start-page: 10039 year: 1998 ident: ja052887ab00042/ja052887ab00042_1 publication-title: J. Org. Chem. contributor: fullname: Singh S. K. – volume: 38 start-page: 14237 year: 1999 ident: ja052887ab00005/ja052887ab00005_1 publication-title: Biochemistry contributor: fullname: Silverman S. K. – volume: 6 start-page: 77 year: 2005 ident: ja052887ab00015/ja052887ab00015_1 publication-title: ChemBioChem contributor: fullname: Köhler O. – volume: 4 start-page: 1128 year: 2003 ident: ja052887ab00001/ja052887ab00001_1 publication-title: ChemBioChem doi: 10.1002/cbic.200300662 contributor: fullname: Wilhelm J. – volume: 123 start-page: 809 year: 2001 ident: ja052887ab00016/ja052887ab00016_1 publication-title: J. Am. Chem. Soc. doi: 10.1021/ja002294u contributor: fullname: Svanvik N. – volume: 15 start-page: 646 year: 2004 ident: ja052887ab00027/ja052887ab00027_1 publication-title: Bioconjugate Chem. doi: 10.1021/bc049976h contributor: fullname: Misra A. – volume: 126 start-page: 15331 year: 2004 ident: ja052887ab00049/ja052887ab00049_1 publication-title: J. Am. Chem. Soc. contributor: fullname: Woods K. K. – volume: 1705 start-page: 1707 year: 2005 ident: ja052887ab00045/ja052887ab00045_1 publication-title: Chem. Commun. contributor: fullname: Babu B. R. – volume: 2 start-page: 280 year: 2004 ident: ja052887ab00046/ja052887ab00046_1 publication-title: Org. Biomol. Chem. doi: 10.1039/B313986G contributor: fullname: Wengel J. – volume: 5 start-page: 1633 year: 2004 ident: ja052887ab00003/ja052887ab00003_1 publication-title: ChemBioChem doi: 10.1002/cbic.200400161 contributor: fullname: Nakatani K. – volume: 15 start-page: 374 year: 2001 ident: ja052887ab00024/ja052887ab00024_1 publication-title: Mol. Cell Probes doi: 10.1006/mcpr.2001.0384 contributor: fullname: French D. J. – volume: 24 start-page: 4997 year: 1996 ident: ja052887ab00012/ja052887ab00012_1 publication-title: Nucleic Acids Res. doi: 10.1093/nar/24.24.4992 contributor: fullname: Ishiguro T. – volume: 99 start-page: 17955 year: 1995 ident: ja052887ab00051/ja052887ab00051_1 publication-title: J. Phys. Chem. contributor: fullname: Netzel T. L. – volume: 21 start-page: 81 year: 2003 ident: ja052887ab00064/ja052887ab00064_1 publication-title: Trends Biotechnol. doi: 10.1016/S0167-7799(02)00038-0 contributor: fullname: Petersen M. – volume: 99 start-page: 17472 year: 1995 ident: ja052887ab00054/ja052887ab00054_1 publication-title: J. Phys. Chem. doi: 10.1021/j100048a024 contributor: fullname: Manoharan M. – volume: 104 start-page: 7227 year: 2000 ident: ja052887ab00011/ja052887ab00011_1 publication-title: J. Phys. Chem. B doi: 10.1021/jp000827f contributor: fullname: Petty J. T. – volume: 25 start-page: 2929 year: 1997 ident: ja052887ab00026/ja052887ab00026_1 publication-title: Nucleic Acids Res. doi: 10.1093/nar/25.14.2923 contributor: fullname: Randolph J. B. – volume: 9 start-page: 3471 year: 2003 ident: ja052887ab00017/ja052887ab00017_1 publication-title: Chem. Eur. J. contributor: fullname: Johansson M. K. – volume: 126 start-page: 4827 year: 2004 ident: ja052887ab00034/ja052887ab00034_1 publication-title: J. Am. Chem. Soc. contributor: fullname: Okamoto A. – volume: 69 start-page: 3275 year: 2004 ident: ja052887ab00033/ja052887ab00033_1 publication-title: J. Org. Chem. doi: 10.1021/jo049824f contributor: fullname: Fujimoto K. – volume-title: Highlights in Bioorganic Chemistry: Methods and Applications year: 2004 ident: ja052887ab00007/ja052887ab00007_1 contributor: fullname: Seitz O. – volume: 99 start-page: 2044 year: 1977 ident: ja052887ab00057/ja052887ab00057_1 publication-title: J. Am. Chem. Soc. doi: 10.1021/ja00449a004 contributor: fullname: Kalyanasundaram K. – volume: 17 start-page: 807 year: 1999 ident: ja052887ab00021/ja052887ab00021_1 publication-title: Nat. Biotechnol. doi: 10.1038/11751 contributor: fullname: Whitcombe D. – volume: 290 start-page: 97 year: 2001 ident: ja052887ab00023/ja052887ab00023_1 publication-title: Anal. Biochem. doi: 10.1006/abio.2000.4957 contributor: fullname: Crockett A. O. – volume: 1478 start-page: 1479 year: 2004 ident: ja052887ab00044/ja052887ab00044_1 publication-title: Chem. Commun. contributor: fullname: Hrdlicka P. J. – volume: 281 start-page: 35 year: 2000 ident: ja052887ab00014/ja052887ab00014_1 publication-title: Anal. Biochem. doi: 10.1006/abio.2000.4534 contributor: fullname: Svanvik N. – volume: 40 start-page: 1106 year: 2001 ident: ja052887ab00029/ja052887ab00029_1 publication-title: Angew. Chem. Int. Ed. doi: 10.1002/1521-3773(20010316)40:6<1104::AID-ANIE11040>3.0.CO;2-2 contributor: fullname: Yamana K. – volume: 100 start-page: 13313 year: 2003 ident: ja052887ab00002/ja052887ab00002_1 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.2233244100 contributor: fullname: Bratu D. P. – volume: 25 start-page: 2521 year: 1997 ident: ja052887ab00019/ja052887ab00019_1 publication-title: J. Nucleic Acids Res. doi: 10.1093/nar/25.12.2516 contributor: fullname: Nazarenko I. A. – volume: 59 start-page: 14 year: 2000 ident: ja052887ab00022/ja052887ab00022_1 publication-title: J. Photochem. Photobiol. B doi: 10.1016/S1011-1344(00)00127-5 contributor: fullname: Talavera E. M. – volume: 80 start-page: 974 year: 1976 ident: ja052887ab00052/ja052887ab00052_1 publication-title: J. Phys. Chem. doi: 10.1021/j100550a010 contributor: fullname: Morris J. V. – volume: 32 start-page: 310 year: 1999 ident: ja052887ab00039/ja052887ab00039_1 publication-title: Acc. Chem. Res. doi: 10.1021/ar980051p contributor: fullname: Wengel J. – volume: 455 start-page: 456 year: 1998 ident: ja052887ab00037/ja052887ab00037_1 publication-title: Chem. Commun. contributor: fullname: Singh S. K. – volume: 11 start-page: 467 year: 1990 ident: ja052887ab00060/ja052887ab00060_1 publication-title: J. Comput. Chem. doi: 10.1002/jcc.540110405 contributor: fullname: Mohamadi R. D. – volume: 11 start-page: 2790 year: 2003 ident: ja052887ab00006/ja052887ab00006_1 publication-title: Bioorg. Med. Chem. doi: 10.1016/S0968-0896(03)00227-X contributor: fullname: Mahara A. – volume: 43 start-page: 758 year: 1997 ident: ja052887ab00020/ja052887ab00020_1 publication-title: Clin. Chem. doi: 10.1093/clinchem/43.5.752 contributor: fullname: Gelmini S. – volume: 1092 start-page: 1104 year: 2002 ident: ja052887ab00031/ja052887ab00031_1 publication-title: J. Chem. Soc. Perkin Trans. 1 contributor: fullname: Korshun V. A. – ident: ja052887ab00040/ja052887ab00040_1 doi: 10.1016/S0040-4020(98)00094-5 – volume: 106 start-page: 784 year: 1984 ident: ja052887ab00059/ja052887ab00059_1 publication-title: J. Am. Chem. Soc. doi: 10.1021/ja00315a052 contributor: fullname: Weiner S. J. |
SSID | ssj0004281 |
Score | 2.2443192 |
Snippet | Homogeneous fluorescence assays for detection of nucleic acids are widely used in biological sciences. Typically, probes such as molecular beacons that rely on... |
Source | Web of Science |
SourceID | proquest crossref pubmed pascalfrancis webofscience istex acs |
SourceType | Aggregation Database Index Database Enrichment Source Publisher |
StartPage | 13293 |
SubjectTerms | Analytical, structural and metabolic biochemistry Biological and medical sciences Chemistry Chemistry, Multidisciplinary DNA - analysis Fundamental and applied biological sciences. Psychology General aspects, investigation methods Microscopy, Fluorescence - methods Molecular Structure Nucleic acids Nucleic Acids - analysis Oligonucleotide Probes - chemistry Oligonucleotides Oligonucleotides, Antisense - chemical synthesis Oligonucleotides, Antisense - chemistry Physical Sciences Pyrenes - chemistry RNA - analysis Science & Technology Temperature |
Title | Multilabeled Pyrene-Functionalized 2‘-Amino-LNA Probes for Nucleic Acid Detection in Homogeneous Fluorescence Assays |
URI | http://dx.doi.org/10.1021/ja052887a https://api.istex.fr/ark:/67375/TPS-0XT1PH2B-C/fulltext.pdf http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestApp=WOS&DestLinkType=FullRecord&UT=000232170800060 https://www.ncbi.nlm.nih.gov/pubmed/16173760 https://search.proquest.com/docview/68610627 |
Volume | 127 |
WOS | 000232170800060 |
WOSCitedRecordID | wos000232170800060 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1Lb9QwELaq9gAXypsUKBZU3Fwl3iSOj0vKaoVgtVK30t4iP6WoNKk22Yr2xM_o7-svYexsuq1YHtfETpSZz5rPmZnPCB1oFyN1TIlWMiQx15IIGSYkBvLOdCoy5iXzv03S8Un8ZZ7Mt9CHP2TwqdMHChMKSwFI0A51lYOO_-TH6-ZHmkU9x2VZOujlg-5OdaFHNfdCz46z4g9XCikasIbtjrHYxDM3hiQffka76Khv4umqTk4Pl608VFe_azr-7cseo0cr-omHHV6eoC1TPUUP8v7Ut2fownfkAjQgHGk8vXSKl2QEwa_7Z1hewVV68_OaDM_KqiZfJ0M8dR1FDQb2iydOHblUeKhKjY9M6-u8KlxWeFyf1YBVUy8bPPq-rBdeRkoZDPgQl81zdDL6PMvHZHU2AxFxRFsipQWuIjlXmdCMW2VDq7WKIhMrqbhKhKt6o0COIikSyqjlocpUqjSX3AgzeIG2q7oyrxBmXNswk4xpmcJ2LROGDlRsleEDHSttA7QPzitWa6spfNqcwralN1-A3vd-Lc47jY5Ngz56j9-OEItTV9TGkmI2PS7C-SyajumnIofX3YPE-pEM8AsEM0DveowU4ByXYBHefEWaARlNKQvQyw4667lAFF35UYAO7mLp9r7XHYLdoePvoRsW_c-wfCXj7uQL2r1_mek1euh1Z11KLXuDttvF0rwFRtXKfb-ifgHIXhp_ |
link.rule.ids | 315,786,790,2782,27109,27957,27958,57093,57143 |
linkProvider | American Chemical Society |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3LbpwwFLWqZJFu-n6QtolVRd05Ag9gvJzSjmg7GY2UiTQ75BcSSgPVwERNVv2Mfl-_pNcGMkk1UrOFawPXx7rn4utjhI60jZE6pEQr6ZOQa0mE9CMSAnlnOhYJc5L5J7M4Owu_LqNlL5Nj98LASzTQU-MW8TfqAlYmyI8ozAjgQrsRgzzc0qD0dLMHkibBQHVZEo8GFaHbTW0EUs2dCLRrnfnTVkSKBpxSdKdZbKObWyOTi0KTx91xRu79XfHJ-fG6lcfq-h9px_t94BP0qCejeNyh5yl6YKpnaC8dzoB7ji7d_lwACgQnjedXVv-STCAUdn8Qy2u4Sv_8-k3GF2VVk-lsjOd2f1GDgQvjmdVKLhUeq1LjT6Z1VV8VLiuc1Rc1INfU6wZPvq_rlROVUgYDWsRV8wKdTT4v0oz0JzUQEQa0JVIWwFwk5yoRmvFCFX6htQoCEyqpuIqErYGjQJUCKSLKaMF9lahYaS65EWb0Eu1UdWVeI8y4LvxEMqZlDMlbIgwdqbBQho90qHThoQPwXt7PtCZ3i-gUkpjBfR56Pwxv_qNT7Nhm9MEN_I2FWJ3bEjcW5Yv5ae4vF8E8ox_zFB53BxmbLhmgGeimhw4HqOQwOHa5RTj35XEC1DSmzEOvOgRt2gJttMVIHjq6Damb-06FCHJFy-Z9axbcxyztRd2tmEG7_z83HaK9bHEyzadfZt_eoIdOkdYutiVv0U67Wpt3wLVaeeAm2V_R4CLq |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3LbpwwFLWqRGq76ftBH4lVRd05Ag9gWE5J0bRNpyNlIs0O-SmhNBANTNVk1c_o9_VLem1gJqlGarZgA74-V_cYX5-L0IGyMVKFlCgpfBKmShAu_IiEQN6ZinnCnGT-12k8OQ0_L6JFv1C0Z2HgIxp4UuM28a1XXyjTKwxYqSA_ouAVwId2I1u421Kh7GRzDpImwUB3WRKPBiWh611tFJLNjSi0aw3602ZF8gYMY7qKFtso59bo5CJR_hB9W4_BJaCcHa5acSiv_pF3vP0gH6EHPSnF4w5Fj9EdXT1B97KhFtxT9MOd0wXAQJBSeHZpdTBJDiGx-5NYXsFV-ufXbzI-L6uaHE_HeGbPGTUYODGeWs3kUuKxLBU-0q3L_qpwWeFJfV4DgnW9anD-fVUvnbiU1BhQwy-bZ-g0_zjPJqSv2EB4GNCWCGGAwYg0lQlXLDXS-EYpGQQ6lEKmMuI2F44CZQoEjyijJvVlImOpUpFqrkfP0U5VV_olwixVxk8EY0rEsIhLuKYjGRqp05EKpTIe2gMLFr3HNYXbTKewmBnM56F3wxQXF51yx7ZG793kr1vw5ZlNdWNRMZ-dFP5iHswm9EORwetuoGPzSMBhCLTTQ_sDXAqYHLvtwp35ijgBihpT5qEXHYo2fYE-2qQkDx1ch9X6vlMjgjWjZfW-bRbcplnWi7tbUYP21f_MtI_uzo7y4vjT9MtrdN8J09o9t-QN2mmXK_0WKFcr9pyf_QULNSVk |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Multilabeled+pyrene-functionalized+2%27-amino-LNA+probes+for+nucleic+acid+detection+in+homogeneous+fluorescence+assays&rft.jtitle=Journal+of+the+American+Chemical+Society&rft.au=HRDLICKA%2C+Patrick+J&rft.au=BABU%2C+B.+Ravindra&rft.au=S%C3%98RENSEN%2C+Mads+D&rft.au=HARRIT%2C+Niels&rft.date=2005-09-28&rft.pub=American+Chemical+Society&rft.issn=0002-7863&rft.eissn=1520-5126&rft.volume=127&rft.issue=38&rft.spage=13293&rft.epage=13299&rft_id=info:doi/10.1021%2Fja052887a&rft.externalDBID=n%2Fa&rft.externalDocID=17144551 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0002-7863&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0002-7863&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0002-7863&client=summon |