Investigation of Biosynthetic Precursors of 3‑Isobutyl-2-Methoxypyrazine Using Stable Isotope Labeling Studies in Bell Pepper Fruits (Capsicum annuum L.)

• Published in: Journal of agricultural and food chemistry Vol. 70; no. 22; pp. 6719 - 6725
• Main Authors: Zamolo, Francesca, Wüst, Matthias
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 08.06.2022; Amer Chemical Soc
• Subjects: Agriculture; Agriculture, Multidisciplinary; biosynthesis; Capsicum annuum; Chemistry; Chemistry and Biology of Aroma and Taste; Chemistry, Applied; comprehensive two-dimensional gas chromatography; food chemistry; Food Science & Technology; glyoxylic acid; headspace analysis; isotope labeling; leucine; Life Sciences & Biomedicine; pericarp; Physical Sciences; Science & Technology; solid phase microextraction; spectrometers; stable isotopes; sweet peppers; Capsicum annuum; 3-alkyl-2-methoxypyrazine (MP); time-of-flight mass spectrometry (ToF-MS); comprehensive two-dimensional gas chromatography (GC×GC); headspace solid-phase microextraction (HS-SPME); CATABOLISM; ESTERS; comprehensive two-dimensional gas chromatography (GCxGC); BRANCHED-CHAIN; FLAVOR; CONSTITUENTS

The biosynthesis of 3-isobutyl-2-methoxypyrazine (IBMP) in bell pepper fruits (Capsicum annuum L.) was investigated by in vivo feeding experiments with stable isotope-labeled precursors. Volatiles were extracted using headspace solid-phase microextraction (HS-SPME) and analyzed by comprehensive two-...