UHPLC-HRMS Spectrometric Analysis: Method Validation and Plasma and Urinary Metabolite Identification after Mango Pulp Intake

• Published in: Journal of agricultural and food chemistry Vol. 71; no. 30; pp. 11520 - 11533
• Main Authors: Cáceres-Jiménez, Salud, Rodríguez-Solana, Raquel, Dobani, Sara, Pourshahidi, Kirsty, Gill, Chris, Moreno-Rojas, José Manuel, Almutairi, Tahani M., Crozier, Alan, Pereira-Caro, Gema
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 02.08.2023
• Subjects: Bioactive Constituents, Metabolites, and Functions; bioavailability; food chemistry; hippuric acid; humans; mango pulp; mangoes; mass spectrometry; metabolites; polyphenols; urine; method validation; mango polyphenols; urinary excretion; metabolites; UHPLC-HRMS analysis

After an acute intake of 300 g of mango purée by 10 subjects, 0 and 24 h urine and plasma samples were analyzed by high-performance liquid chromatography–high-resolution mass spectrometry. The method was first validated for 44 reference polyphenols in terms of linearity, specificity, limits of dete...