UHPLC-HRMS Spectrometric Analysis: Method Validation and Plasma and Urinary Metabolite Identification after Mango Pulp Intake

• Published in: Journal of agricultural and food chemistry Vol. 71; no. 30; pp. 11520 - 11533
• Main Authors: Cáceres-Jiménez, Salud, Rodríguez-Solana, Raquel, Dobani, Sara, Pourshahidi, Kirsty, Gill, Chris, Moreno-Rojas, José Manuel, Almutairi, Tahani M., Crozier, Alan, Pereira-Caro, Gema
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 02.08.2023
• Subjects: Bioactive Constituents, Metabolites, and Functions; bioavailability; food chemistry; hippuric acid; humans; mango pulp; mangoes; mass spectrometry; metabolites; polyphenols; urine; method validation; mango polyphenols; urinary excretion; metabolites; UHPLC-HRMS analysis
• ISSN: 0021-8561; 1520-5118; 1520-5118
• DOI: 10.1021/acs.jafc.3c03846

After an acute intake of 300 g of mango purée by 10 subjects, 0 and 24 h urine and plasma samples were analyzed by high-performance liquid chromatography–high-resolution mass spectrometry. The method was first validated for 44 reference polyphenols in terms of linearity, specificity, limits of detection and quantification, intra-day and inter-day precision, recovery, and matrix effects in two biological matrices. After method validation, a total of 94 microbial-derived phenolic catabolites, including 15 cinnamic acids, 3 phenylhydracrylic acids, 14 phenylpropanoic acids, 12 phenylacetic acids, 28 benzoic acids, 2 mandelic acids, 15 hydroxybenzenes, and 5 hippuric acid derivatives, were identified or tentatively identified in urine and/or plasma. These results establish the value of the UHPLC-HRMS protocol and the use of authentic standards to obtain a detailed and accurate picture of mango polyphenol metabolites, together with their phase II conjugated metabolites, in human bioavailability studies.