Biosynthesis of intestinal microvillar proteins. Dimerization of aminopeptidase N and lactase-phlorizin hydrolase

• Published in: Biochemistry (Easton) Vol. 29; no. 1; pp. 305 - 308
• Main Author: Danielsen, E. Michael
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 09.01.1990
• Subjects: AMINO ACIDS; AMINOPEPTIDASES; Aminopeptidases - biosynthesis; Analytical, structural and metabolic biochemistry; ANIMALS; BASIC BIOLOGICAL SCIENCES; BETA DECAY RADIOISOTOPES; beta-Galactosidase - biosynthesis; BETA-MINUS DECAY RADIOISOTOPES; Biological and medical sciences; BIOSYNTHESIS; BODY; CARBOXYLIC ACIDS; CD13 Antigens; CELL CONSTITUENTS; CHEMICAL REACTIONS; CROSS-LINKING; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DIMERIZATION; Dimethyl Adipimidate - metabolism; DOMESTIC ANIMALS; DRUGS; ELECTROPHORESIS; ENZYMES; Enzymes and enzyme inhibitors; EVEN-ODD NUCLEI; Fundamental and applied biological sciences. Psychology; Galactosidases - biosynthesis; GASTROINTESTINAL TRACT; Glucosidases - biosynthesis; GLYCOPROTEINS; GLYCOSYL HYDROLASES; HYDROLASES; Intestinal Mucosa - enzymology; Intestinal Mucosa - ultrastructure; INTESTINES; ISOTOPES; Kinetics; Lactase-Phlorizin Hydrolase - biosynthesis; LIGHT NUCLEI; LIPOTROPIC FACTORS; MAMMALS; MEMBRANES; METHIONINE; Microvilli - enzymology; MUCOUS MEMBRANES; NUCLEI; O-GLYCOSYL HYDROLASES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; ORGANS; PEPTIDE HYDROLASES; POLYMERIZATION; Protein Processing, Post-Translational; PROTEINS; RADIOISOTOPES; SULFUR 35; SULFUR ISOTOPES; SWINE; SYNTHESIS; VERTEBRATES 550201 > Biochemistry > Tracer Techniques; Radiolabelling; Gel electrophoresis; Enzyme; Mucosa; Gut; Explant; Biosynthesis; Pig; Vertebrata; Brush border; Mammalia; Artiodactyla; Kinetics; Densitometry; Aminopeptidase; Immunoelectrophoresis; Localization; Dimerization; Ungulata

The pig intestinal brush border enzymes aminopeptidase N (EC 3.4.11.2) and lactase-phlorizin hydrolase (EC 3.2.1.23-62) are present in the microvillar membrane as homodimers. Dimethyl adipimidate was used to cross-link the two [35S]methionine-labeled brush border enzymes from cultured mucosal explan...