Production of Site-Specific Antibody–Drug Conjugates Using Optimized Non-Natural Amino Acids in a Cell-Free Expression System

• Published in: Bioconjugate chemistry Vol. 25; no. 2; pp. 351 - 361
• Main Authors: Zimmerman, Erik S, Heibeck, Tyler H, Gill, Avinash, Li, Xiaofan, Murray, Christopher J, Madlansacay, Mary Rose, Tran, Cuong, Uter, Nathan T, Yin, Gang, Rivers, Patrick J, Yam, Alice Y, Wang, Willie D, Steiner, Alexander R, Bajad, Sunil U, Penta, Kalyani, Yang, Wenjin, Hallam, Trevor J, Thanos, Christopher D, Sato, Aaron K
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 19.02.2014; Amer Chemical Soc
• Subjects: Amino acids; Amino Acids - chemistry; Antigens; Biochemical Research Methods; Biochemistry & Molecular Biology; Cell Line; Cell-Free System; Chemistry; Chemistry, Multidisciplinary; Chemistry, Organic; Chromatography, Liquid; Cytotoxicity; Gene expression; Genetics; High-Throughput Screening Assays; Humans; Immunoconjugates - chemistry; Immunoconjugates - pharmacology; Immunogenicity; Life Sciences & Biomedicine; Molecules; Pharmacokinetics; Physical Sciences; Protein synthesis; Science & Technology; Tandem Mass Spectrometry; Tumors; GENETIC-CODE; EVOLUTION; EFFICACY; TRANSFER-RNA SYNTHETASE; STABILITY; CYTOTOXIC DRUG; COPPER-FREE; PROTEINS; CANCER; PAIR

Antibody–drug conjugates (ADCs) are a targeted chemotherapeutic currently at the cutting edge of oncology medicine. These hybrid molecules consist of a tumor antigen-specific antibody coupled to a chemotherapeutic small molecule. Through targeted delivery of potent cytotoxins, ADCs exhibit improved...