Cell-Membrane-Localizing, Two-Photon Probe for Ratiometric Imaging of γ‑Glutamyl Transpeptidase in Cancerous Cells and Tissues

• Published in: Analytical chemistry (Washington) Vol. 92; no. 18; pp. 12678 - 12685
• Main Authors: Reo, Ye Jin, Dai, Mingchong, Yang, Yun Jae, Ahn, Kyo Han
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 15.09.2020
• Subjects: analytical chemistry; Animals; Biotechnology; Cancer; Cell Membrane - enzymology; Cell surface; Cells, Cultured; Chemistry; Colon; Enzymatic activity; Enzyme activity; Enzymes; fluorescence; fluorescent dyes; Fluorescent Dyes - chemistry; Fluorescent indicators; gamma-Glutamyltransferase - analysis; gamma-Glutamyltransferase - metabolism; Humans; Kidneys; liver; lungs; Medical imaging; Membranes; Mice; Mice, Inbred BALB C; Microscopy, Fluorescence; neoplasm cells; neoplasms; Optical Imaging; Organs; Pancreas; Photons; proteinases; quantitative analysis; Reliability analysis; Spleen; stomach; Thigh; Tissues; Tumor cell lines; Tumors; Xenografts; Xenotransplantation; γ-Glutamyltransferase

γ-Glutamyl transpeptidase (GGT), a cell surface-bound protease, is associated with various diseases including cancer. The detection of the enzyme activity is an important subject, leading to about 40 activatable fluorescent probes so far. All of them, however, lack the membrane-localizing ability, r...