Versatile Electroanalytical Bioplatforms for Simultaneous Determination of Cancer-Related DNA 5‑Methyl- and 5‑Hydroxymethyl-Cytosines at Global and Gene-Specific Levels in Human Serum and Tissues

• Published in: ACS sensors Vol. 4; no. 1; pp. 227 - 234
• Main Authors: Povedano, Eloy, Montiel, Víctor Ruiz-Valdepeñas, Valverde, Alejandro, Navarro-Villoslada, Fernando, Yáñez-Sedeño, Paloma, Pedrero, María, Montero-Calle, Ana, Barderas, Rodrigo, Peláez-García, Alberto, Mendiola, Marta, Hardisson, David, Feliú, Jaime, Camps, Jordi, Rodríguez-Tomàs, Elisabet, Joven, Jorge, Arenas, Meritxell, Campuzano, Susana, Pingarrón, José M
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 25.01.2019
• Subjects: 5-Methylcytosine - analogs & derivatives; 5-Methylcytosine - blood; 5-Methylcytosine - immunology; Antibodies, Monoclonal - immunology; Armoracia - enzymology; Biomarkers, Tumor - blood; Biomarkers, Tumor - chemistry; Biomarkers, Tumor - immunology; Biosensing Techniques - methods; DNA - blood; DNA - chemistry; DNA - immunology; DNA Methylation; DNA Modification Methylases - genetics; DNA Repair Enzymes - genetics; Electrochemical Techniques - methods; Fluorescent Dyes - chemistry; Horseradish Peroxidase - chemistry; Humans; Hydrogen Peroxide - chemistry; Immunomagnetic Separation; Limit of Detection; Tumor Suppressor Proteins - genetics; 5-mC; 5-hmC; paraffin-embedded colorectal tissues; global and gene-specific DNA methylation; cancer; human serum; electrochemical platforms

This paper reports the preparation of versatile electrochemical biosensing platforms for the simple, rapid, and PCR-independent detection of the most frequent DNA methylation marks (5-methylcytosine, 5-mC, and/or 5-hydroxymethylcytosine, 5-hmC) both at global and gene-specific levels. The implemente...