Simultaneous Use of Time-Resolved Fluorescence and Anti-Stokes Photoluminescence in a Bioaffinity Assay

• Published in: Analytical chemistry (Washington) Vol. 77; no. 9; pp. 2826 - 2834
• Main Authors: Kuningas, Katri, Rantanen, Terhi, Karhunen, Ulla, Lövgren, Timo, Soukka, Tero
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.05.2005
• Subjects: Analytical chemistry; Biological Assay - methods; Biomedical research; Biotechnology; Chemistry; Exact sciences and technology; Fluorescence; Fluorometry - methods; Immunoassay; Lanthanoid Series Elements - chemistry; Light; Luminescent Measurements - methods; Serum Albumin, Bovine - analysis; Spectrometric and optical methods; Time resolution; Lifetime; Lanthanide; Chemical analysis; Detection limit; Linearity; Photoluminescence; Anti Stokes line; Solid phase; Optical frequency conversion; Europium; Fluorescence spectrometry

A bioaffinity assay is described where anti-Stokes photoluminescence of inorganic lanthanide phosphors and time-resolved fluorescence of lanthanide chelates are measured from a single microtitration well without any disturbance from these label technologies to each other. Up-converting phosphor (UPC...