Chemical Modification of M13 Bacteriophage and Its Application in Cancer Cell Imaging

• Published in: Bioconjugate chemistry Vol. 21; no. 7; pp. 1369 - 1377
• Main Authors: Li, Kai, Chen, Yi, Li, Siqi, Nguyen, Huong Giang, Niu, Zhongwei, You, Shaojin, Mello, Charlene M, Lu, Xiaobing, Wang, Qian
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 21.07.2010
• Subjects: Bacteria; Bacteriophage M13 - chemistry; Cancer; Carboxylic acids; Cells; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; Folic acid; Folic Acid - chemistry; HeLa Cells; Humans; Liquid chromatography; Medical imaging; Molecular Imaging - methods; Molecular Structure; Molecules; Neoplasms - pathology; Particle Size; Phenols; Stereoisomerism; Surface Properties; Tumor Cells, Cultured; Vitamin B
• ISSN: 1043-1802; 1520-4812; 1520-4812
• DOI: 10.1021/bc900405q

The M13 bacteriophage has been demonstrated to be a robust scaffold for bionanomaterial development. In this paper, we report on the chemical modifications of three kinds of reactive groups, i.e., the amino groups of lysine residues or N-terminal, the carboxylic acid groups of aspartic acid or gluta...