Column Characterization and Selection Systems in Reversed-Phase High-Performance Liquid Chromatography

• Published in: Chemical reviews Vol. 119; no. 6; pp. 3674 - 3729
• Main Authors: Žuvela, Petar, Skoczylas, Magdalena, Jay Liu, J, Ba̧czek, Tomasz, Kaliszan, Roman, Wong, Ming Wah, Buszewski, Bogusław
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 27.03.2019
• Subjects: automation; Chromatography; Computer simulation; End users; High performance liquid chromatography; human resources; hydrogen bonding; Hydrogen bonds; ligands; Liquid chromatography; Molecular dynamics; Pore size; Porosity; reversed-phase high performance liquid chromatography; Selectivity; Separation

Reversed-phase high-performance liquid chromatography (RP-HPLC) is the most popular chromatographic mode, accounting for more than 90% of all separations. HPLC itself owes its immense popularity to it being relatively simple and inexpensive, with the equipment being reliable and easy to operate. Due...