Fluorescence Quenching of (Dimethylamino)naphthalene Dyes Badan and Prodan by Tryptophan in Cytochromes P450 and Micelles

• Published in: The journal of physical chemistry. B Vol. 118; no. 34; pp. 10085 - 10091
• Main Authors: Pospíšil, Petr, Luxem, Katja E, Ener, Maraia, Sýkora, Jan, Kocábová, Jana, Gray, Harry B, Vlček, Antonín, Hof, Martin
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 28.08.2014
• Subjects: 2-Naphthylamine - analogs & derivatives; 2-Naphthylamine - chemistry; Archaeal Proteins - chemistry; Bacterial Proteins - chemistry; Cytochrome P-450 Enzyme System - chemistry; Fluorescence; Fluorescent Dyes - chemistry; Kinetics; Micelles; Models, Molecular; NADPH-Ferrihemoprotein Reductase - chemistry; Spectrometry, Fluorescence; Tryptophan - chemistry; Water

Fluorescence of 2-(N,N-dimethylamino)-6-propionylnaphthalene dyes Badan and Prodan is quenched by tryptophan in Brij 58 micelles as well as in two cytochrome P450 proteins (CYP102, CYP119) with Badan covalently attached to a cysteine residue. Formation of nonemissive complexes between a dye molecule...