Addressing Statistical Biases in Nucleotide-Derived Protein Databases for Proteogenomic Search Strategies

• Published in: Journal of proteome research Vol. 11; no. 11; pp. 5221 - 5234
• Main Authors: Blakeley, Paul, Overton, Ian M, Hubbard, Simon J
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 02.11.2012
• Subjects: Base Sequence; Databases, Protein; Expressed Sequence Tags; Genomics; Mass Spectrometry; Nucleotides - chemistry; Probability; Proteomics; peptide spectrum match; posterior error probability; expressed sequence tag; proteogenomics; false discovery rate

Proteogenomics has the potential to advance genome annotation through high quality peptide identifications derived from mass spectrometry experiments, which demonstrate a given gene or isoform is expressed and translated at the protein level. This can advance our understanding of genome function, di...