Combining Avidin−Biotin Chemistry with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

• Published in: Analytical chemistry (Washington) Vol. 68; no. 19; pp. 3382 - 3387
• Main Authors: Schriemer, David C, Li, Liang
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.10.1996
• Subjects: Analytical biochemistry: general aspects, technics, instrumentation; Analytical, structural and metabolic biochemistry; Avidin - chemistry; Biological and medical sciences; Biotin - chemistry; Chemistry; Fundamental and applied biological sciences. Psychology; Molecular Structure; Scientific imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Biochemical analysis; Vitamin; Glycoproteins; Biotin; Mass spectrometry; Avidin; Laser desorption; Chemical ionization

Molecular recognition based on the high affinity of avidin for biotin has been combined with matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The rich chemistry of biotinylation reagents underlies the development of a mass tracer method for the rapid and sensitive analysis of b...