Kinetic Measurements on Single-Molecule Disulfide Bond Cleavage

• Published in: Journal of the American Chemical Society Vol. 133; no. 10; pp. 3528 - 3534
• Main Authors: Liang, Jian, Fernández, Julio M
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 16.03.2011
• Subjects: Disulfides - chemistry; Dithiothreitol - chemistry; Kinetics; Phosphines - chemistry; Protein Folding; Proteins - chemistry; Spectrum Analysis - methods

We use single-molecule force clamp spectroscopy (SMFCS) to explore the reactivity of tris(2-carboxyethyl)phosphine (TCEP), 1, 4-dl-dithiothreitol (DTT) and hydrosulfide anion (HS−) on disulfide bonds within a mechanically stretched polypeptide. The single-bond level bimolecular nucleophilic substitu...