Pulsed Stable Isotope Labeling of Amino Acids in Cell Culture Uncovers the Dynamic Interactions between HIV-1 and the Monocyte-Derived Macrophage

• Published in: Journal of proteome research Vol. 10; no. 6; pp. 2852 - 2862
• Main Authors: Kraft-Terry, Stephanie D, Engebretsen, Ian L, Bastola, Dhundy K, Fox, Howard S, Ciborowski, Pawel, Gendelman, Howard E
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 03.06.2011
• Subjects: Amino Acids - metabolism; Cell Culture Techniques; Cells, Cultured; Gene Expression Regulation; HIV Core Protein p24 - genetics; HIV Core Protein p24 - metabolism; HIV-1 - genetics; HIV-1 - physiology; Humans; Interferons - metabolism; Isotope Labeling; Macrophages - metabolism; Macrophages - virology; Monocytes - cytology; Proteome - genetics; Proteome - metabolism; Signal Transduction; Time Factors; cell function; human immunodeficiency virus; pulsed stable isotope labeling of amino acids in cell culture; monocyte-derived macrophages; proteome

Dynamic interactions between human immunodeficiency virus-1 (HIV-1) and the macrophage govern the tempo of viral dissemination and replication in its human host. HIV-1 affects macrophage phenotype, and the macrophage, in turn, can modulate the viral life cycle. While these processes are linked to ho...