Compositional Analysis of Glyphosate-Tolerant Soybeans Treated with Glyphosate

• Published in: Journal of agricultural and food chemistry Vol. 47; no. 10; pp. 4469 - 4473
• Main Authors: Taylor, Nancy B, Fuchs, Roy L, MacDonald, John, Shariff, Ahmed R, Padgette, Stephen R
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.10.1999
• Subjects: 5-enolpyruvylshikimate-3-phosphate synthase; Agronomy. Soil science and plant productions; alkyl (aryl) transferases; amino acids; Amino Acids - analysis; ash; Biological and medical sciences; Biotechnology; carbohydrates; chemical composition; Consumer Product Safety; Enzyme-Linked Immunosorbent Assay; Estrogens, Non-Steroidal - analysis; fiber content; Fundamental and applied biological sciences. Psychology; Genetic engineering; genetic resistance; Genetic technics; Genetics and breeding of economic plants; Glycine - analogs & derivatives; Glycine - analysis; Glycine - pharmacology; Glycine max; Glycine max - chemistry; Glycine max - drug effects; Glycine max - genetics; Glyphosate; herbicide resistance; Herbicides - analysis; Herbicides - pharmacology; Humans; Isoflavones; Methods. Procedures. Technologies; Pest resistance; Phytoestrogens; plant estrogens; Plant Preparations; plant proteins; proximate composition; soybean oil; soybeans; Transgenic animals and transgenic plants; Transgenic plants; Varietal selection. Specialized plant breeding, plant breeding aims; Weeds; Biochemical analysis; Pesticides; Tolerance; Biosynthesis; Soybean; Experimental study; PROXIMATE COMPOSITION; Glyphosate; Isoflavone derivatives; Herbicide; Grain legume; Chemical treatment; Aminoacid; Genetic engineering; Genetically modified organism; Comparative study
• DOI: 10.1021/jf990056g
• ISSN: 0021-8561

The compositional analyses and safety assessment of glyphosate-tolerant soybeans (GTS) were previously described. These analyses were extensive and included addressing the potential effects on seed composition from the genetic modification. Detailed compositional analyses established that GTS, which had not been treated with glyphosate, were comparable to the parental soybean line and to other conventional soybeans. In this study, two GTS lines, 40-3-2 and 61-67-1, were treated with commercial levels of glyphosate, the active ingredient in Roundup herbicide. The composition of the seed from soybeans sprayed with glyphosate was compared to that of a nonsprayed parental control cultivar, A5403. The nutrients measured in the seed included protein, oil, ash, fiber, carbohydrates, and amino acids. The concentration of isoflavones (also referred to as phytoestrogens) was also measured as these compounds are derived from the same biochemical pathway that was engineered for glyphosate tolerance. The analytical results from these studies demonstrate that the GTS soybeans treated with glyphosate were comparable to the parental soybean cultivar, A5403, and other conventional soybean varieties. Keywords: Soybean; glyphosate-tolerant; compositional analysis; glyphosate