Conformational Heterogeneity and Quasi-Static Self-Quenching in DNA Containing a Fluorescent Guanine Analogue, 3MI or 6MI

• Published in: Biochemistry (Easton) Vol. 48; no. 37; pp. 8861 - 8868
• Main Authors: Wojtuszewski Poulin, Kristi, Smirnov, Aleksandr V, Hawkins, Mary E, Balis, Frank M, Knutson, Jay R
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 22.09.2009
• Subjects: Deoxyguanosine - analogs & derivatives; Deoxyguanosine - chemistry; DNA Probes - chemistry; DNA, Single-Stranded - chemistry; Fluorescence Polarization; Guanine - analogs & derivatives; Nucleic Acid Conformation; Nucleic Acid Heteroduplexes - chemical synthesis; Spectrometry, Fluorescence; Static Electricity; Xanthopterin - analogs & derivatives; Xanthopterin - chemistry

Two different microenvironments in the DNA sequence 5′-act aGa gat ccc tca gac cct ttt agt cag tGt gga-3′ (in both single- and double-stranded forms) are explored using two similar fluorescent nucleoside analogues, 3MI and 6MI. Each probe was evaluated in two environments, one strand with the probe...