Cytoplasmic Tail Truncation of SARS-CoV-2 Spike Protein Enhances Titer of Pseudotyped Vectors but Masks the Effect of the D614G Mutation

• Published in: Journal of virology Vol. 95; no. 22; p. e0096621
• Main Authors: Chen, Hsu-Yu, Huang, Chun, Tian, Lu, Huang, Xiaoli, Zhang, Chennan, Llewellyn, George N, Rogers, Geoffrey L, Andresen, Kevin, O’Gorman, Maurice R. G, Chen, Ya-Wen, Cannon, Paula M
• Format: Journal Article
• Language: English
• Published: 1752 N St., N.W., Washington, DC American Society for Microbiology 27.10.2021
• Subjects: Structure and Assembly; Virology; D614G mutation; SARS-CoV-2; Spike protein; cytoplasmic tail truncation; pseudotyped vectors

The high pathogenicity of SARS-CoV-2 requires it to be handled under biosafety level 3 conditions. Consequently, Spike protein-pseudotyped vectors are a useful tool to study viral entry and its inhibition, with retroviral, lentiviral (LV), and vesicular stomatitis virus (VSV) vectors the most common...