Synthesis and Evolution of a Threose Nucleic Acid Aptamer Bearing 7‑Deaza-7-Substituted Guanosine Residues

• Published in: Journal of the American Chemical Society Vol. 140; no. 17; pp. 5706 - 5713
• Main Authors: Mei, Hui, Liao, Jen-Yu, Jimenez, Randi M, Wang, Yajun, Bala, Saikat, McCloskey, Cailen, Switzer, Christopher, Chaput, John C
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 02.05.2018; Amer Chemical Soc
• Subjects: Chemistry; Chemistry, Multidisciplinary; Physical Sciences; Science & Technology; SYSTEM; HALOGEN BONDS; BASE-PAIRS; REPLICATION; GENETIC POLYMERS; RNA; TNA; TRANSCRIPTION; IN-VITRO SELECTION; DNA-POLYMERASE

In vitro selection experiments carried out on artificial genetic polymers require robust and faithful methods for copying genetic information back and forth between DNA and xeno-nucleic acids (XNA). Previously, we have shown that Kod-RI, an engineered polymerase developed to transcribe DNA templates...