Genetic diversity and virulence of Bacillus cereus group isolates from bloodstream infections
This study provides novel insights into the genetic diversity and virulence potential of B. cereus strains causing bloodstream infections in a Japanese hospital setting. These findings suggest diverse infection pathways and highlight the importance of continuous molecular epidemiological surveillanc...
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| Published in | Microbiology spectrum Vol. 13; no. 3; p. e0240724 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
American Society for Microbiology
04.03.2025
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| Online Access | Get full text |
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| Abstract | This study provides novel insights into the genetic diversity and virulence potential of B. cereus strains causing bloodstream infections in a Japanese hospital setting. These findings suggest diverse infection pathways and highlight the importance of continuous molecular epidemiological surveillance for effective infection control. |
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| AbstractList | This study provides novel insights into the genetic diversity and virulence potential of B. cereus strains causing bloodstream infections in a Japanese hospital setting. These findings suggest diverse infection pathways and highlight the importance of continuous molecular epidemiological surveillance for effective infection control. catheter-related bloodstream infections (CRBSIs) are an increasing concern in Japanese hospitals. Although their clinical characteristics have been explored, the genetic relationships and virulence profiles of isolates from CRBSIs remain understudied. Here, using advanced genomic techniques, we investigated the genetic diversity, phylogenetic relationships, and virulence profiles of isolates from patients with bloodstream infections. We analyzed 28 group strains isolated from blood samples at the University of Tokyo Hospital between 2005 and 2017 using whole-genome sequencing, core-genome single-nucleotide polymorphism (SNP) typing, and virulence gene profiling. Core-genome SNP analysis revealed significant genetic diversity among the isolates, suggesting multiple independent sources of infection. The isolates predominantly belonged to clades III and IV, with distinct virulence gene profiles. All clade III isolates contained operon genes, whereas four isolates from clade IV harbored cereulide synthetase genes ( ). One isolate possessed a capsule gene operon ( ), a rare finding among clinical strains. Biofilm formation ability was observed in 50% of catheter-related isolates, although this ability was not significantly different from that of the noncatheter-related isolates.IMPORTANCEThis study provides novel insights into the genetic diversity and virulence potential of strains causing bloodstream infections in a Japanese hospital setting. These findings suggest diverse infection pathways and highlight the importance of continuous molecular epidemiological surveillance for effective infection control. Bacillus cereus catheter-related bloodstream infections (CRBSIs) are an increasing concern in Japanese hospitals. Although their clinical characteristics have been explored, the genetic relationships and virulence profiles of B. cereus isolates from CRBSIs remain understudied. Here, using advanced genomic techniques, we investigated the genetic diversity, phylogenetic relationships, and virulence profiles of B. cereus isolates from patients with bloodstream infections. We analyzed 28 B. cereus group strains isolated from blood samples at the University of Tokyo Hospital between 2005 and 2017 using whole-genome sequencing, core-genome single-nucleotide polymorphism (SNP) typing, and virulence gene profiling. Core-genome SNP analysis revealed significant genetic diversity among the isolates, suggesting multiple independent sources of infection. The isolates predominantly belonged to panC clades III and IV, with distinct virulence gene profiles. All panC clade III isolates contained hbl operon genes, whereas four isolates from clade IV harbored cereulide synthetase genes (cesABCD). One isolate possessed a capsule gene operon (capBCADE), a rare finding among clinical B. cereus strains. Biofilm formation ability was observed in 50% of catheter-related isolates, although this ability was not significantly different from that of the noncatheter-related isolates.IMPORTANCEThis study provides novel insights into the genetic diversity and virulence potential of B. cereus strains causing bloodstream infections in a Japanese hospital setting. These findings suggest diverse infection pathways and highlight the importance of continuous molecular epidemiological surveillance for effective infection control. Bacillus cereus catheter-related bloodstream infections (CRBSIs) are an increasing concern in Japanese hospitals. Although their clinical characteristics have been explored, the genetic relationships and virulence profiles of B. cereus isolates from CRBSIs remain understudied. Here, using advanced genomic techniques, we investigated the genetic diversity, phylogenetic relationships, and virulence profiles of B. cereus isolates from patients with bloodstream infections. We analyzed 28 B. cereus group strains isolated from blood samples at the University of Tokyo Hospital between 2005 and 2017 using whole-genome sequencing, core-genome single-nucleotide polymorphism (SNP) typing, and virulence gene profiling. Core-genome SNP analysis revealed significant genetic diversity among the isolates, suggesting multiple independent sources of infection. The isolates predominantly belonged to panC clades III and IV, with distinct virulence gene profiles. All panC clade III isolates contained hbl operon genes, whereas four isolates from clade IV harbored cereulide synthetase genes (cesABCD). One isolate possessed a capsule gene operon (capBCADE), a rare finding among clinical B. cereus strains. Biofilm formation ability was observed in 50% of catheter-related isolates, although this ability was not significantly different from that of the noncatheter-related isolates.IMPORTANCEThis study provides novel insights into the genetic diversity and virulence potential of B. cereus strains causing bloodstream infections in a Japanese hospital setting. These findings suggest diverse infection pathways and highlight the importance of continuous molecular epidemiological surveillance for effective infection control.Bacillus cereus catheter-related bloodstream infections (CRBSIs) are an increasing concern in Japanese hospitals. Although their clinical characteristics have been explored, the genetic relationships and virulence profiles of B. cereus isolates from CRBSIs remain understudied. Here, using advanced genomic techniques, we investigated the genetic diversity, phylogenetic relationships, and virulence profiles of B. cereus isolates from patients with bloodstream infections. We analyzed 28 B. cereus group strains isolated from blood samples at the University of Tokyo Hospital between 2005 and 2017 using whole-genome sequencing, core-genome single-nucleotide polymorphism (SNP) typing, and virulence gene profiling. Core-genome SNP analysis revealed significant genetic diversity among the isolates, suggesting multiple independent sources of infection. The isolates predominantly belonged to panC clades III and IV, with distinct virulence gene profiles. All panC clade III isolates contained hbl operon genes, whereas four isolates from clade IV harbored cereulide synthetase genes (cesABCD). One isolate possessed a capsule gene operon (capBCADE), a rare finding among clinical B. cereus strains. Biofilm formation ability was observed in 50% of catheter-related isolates, although this ability was not significantly different from that of the noncatheter-related isolates.IMPORTANCEThis study provides novel insights into the genetic diversity and virulence potential of B. cereus strains causing bloodstream infections in a Japanese hospital setting. These findings suggest diverse infection pathways and highlight the importance of continuous molecular epidemiological surveillance for effective infection control. Bacillus cereus catheter-related bloodstream infections (CRBSIs) are an increasing concern in Japanese hospitals. Although their clinical characteristics have been explored, the genetic relationships and virulence profiles of B. cereus isolates from CRBSIs remain understudied. Here, using advanced genomic techniques, we investigated the genetic diversity, phylogenetic relationships, and virulence profiles of B. cereus isolates from patients with bloodstream infections. We analyzed 28 B. cereus group strains isolated from blood samples at the University of Tokyo Hospital between 2005 and 2017 using whole-genome sequencing, core-genome single-nucleotide polymorphism (SNP) typing, and virulence gene profiling. Core-genome SNP analysis revealed significant genetic diversity among the isolates, suggesting multiple independent sources of infection. The isolates predominantly belonged to panC clades III and IV, with distinct virulence gene profiles. All panC clade III isolates contained hbl operon genes, whereas four isolates from clade IV harbored cereulide synthetase genes ( cesABCD ). One isolate possessed a capsule gene operon ( capBCADE ), a rare finding among clinical B. cereus strains. Biofilm formation ability was observed in 50% of catheter-related isolates, although this ability was not significantly different from that of the noncatheter-related isolates. ABSTRACT Bacillus cereus catheter-related bloodstream infections (CRBSIs) are an increasing concern in Japanese hospitals. Although their clinical characteristics have been explored, the genetic relationships and virulence profiles of B. cereus isolates from CRBSIs remain understudied. Here, using advanced genomic techniques, we investigated the genetic diversity, phylogenetic relationships, and virulence profiles of B. cereus isolates from patients with bloodstream infections. We analyzed 28 B. cereus group strains isolated from blood samples at the University of Tokyo Hospital between 2005 and 2017 using whole-genome sequencing, core-genome single-nucleotide polymorphism (SNP) typing, and virulence gene profiling. Core-genome SNP analysis revealed significant genetic diversity among the isolates, suggesting multiple independent sources of infection. The isolates predominantly belonged to panC clades III and IV, with distinct virulence gene profiles. All panC clade III isolates contained hbl operon genes, whereas four isolates from clade IV harbored cereulide synthetase genes (cesABCD). One isolate possessed a capsule gene operon (capBCADE), a rare finding among clinical B. cereus strains. Biofilm formation ability was observed in 50% of catheter-related isolates, although this ability was not significantly different from that of the noncatheter-related isolates.IMPORTANCEThis study provides novel insights into the genetic diversity and virulence potential of B. cereus strains causing bloodstream infections in a Japanese hospital setting. These findings suggest diverse infection pathways and highlight the importance of continuous molecular epidemiological surveillance for effective infection control. |
| Author | Tsutsumi, Takeya Okugawa, Shu Moriya, Kyoji Okutani, Akiko Fujimoto, Fumie Ikeda, Mahoko Maeda, Ken |
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| Keywords | epidemiology bloodstream infection Bacillus cereus group whole-genome analysis |
| Language | English |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Akiko Okutani and Shu Okugawa contributed equally to this article. Author order was determined on the basis of seniority. The authors declare no conflict of interest. |
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| References | e_1_3_4_3_2 e_1_3_4_2_2 e_1_3_4_9_2 e_1_3_4_8_2 e_1_3_4_7_2 e_1_3_4_6_2 e_1_3_4_5_2 e_1_3_4_4_2 e_1_3_4_11_2 e_1_3_4_22_2 e_1_3_4_12_2 e_1_3_4_23_2 e_1_3_4_20_2 e_1_3_4_10_2 e_1_3_4_21_2 e_1_3_4_15_2 e_1_3_4_16_2 e_1_3_4_13_2 e_1_3_4_14_2 e_1_3_4_19_2 e_1_3_4_17_2 e_1_3_4_18_2 Dohmae, S, Okubo, T, Higuchi, W, Takano, T, Isobe, H, Baranovich, T, Kobayashi, S, Uchiyama, M, Tanabe, Y, Itoh, M, Yamamoto, T (B6) 2008; 69 Treangen, TJ, Ondov, BD, Koren, S, Phillippy, AM (B17) 2014; 15 Ceuppens, S, Boon, N, Uyttendaele, M (B1) 2013; 84 Sasahara, T, Hayashi, S, Morisawa, Y, Sakihama, T, Yoshimura, A, Hirai, Y (B8) 2011; 30 Stepanović, S, Vuković, D, Hola, V, Di Bonaventura, G, Djukić, S, Cirković, I, Ruzicka, F (B16) 2007; 115 Aoyagi, T, Oshima, K, Endo, S, Baba, H, Kanamori, H, Yoshida, M, Tokuda, K, Kaku, M (B5) 2020; 15 Ikeda, M, Yagihara, Y, Tatsuno, K, Okazaki, M, Okugawa, S, Moriya, K (B10) 2015; 14 Okutani, A, Inoue, S, Noguchi, A, Kaku, Y, Morikawa, S (B14) 2019; 19 Kuroki, R, Kawakami, K, Qin, L, Kaji, C, Watanabe, K, Kimura, Y, Ishiguro, C, Tanimura, S, Tsuchiya, Y, Hamaguchi, I, Sakakura, M, Sakabe, S, Tsuji, K, Inoue, M, Watanabe, H (B7) 2009; 48 Okutani, A, Okugawa, S, Fujimoto, F, Ikeda, M, Tsutsumi, T, Moriya, K, Maeda, K (B15) 2024; 13 Carroll, LM, Wiedmann, M, Kovac, J (B19) 2020; 11 Okamoto, A, Okutani, A, Yi-Wei Tang, MH, Dongyou, L, Sails, A, Spearman, P, Zhang, JR (B2) 2024 Carroll, LM, Cheng, RA, Kovac, J (B20) 2020; 11 Guinebretière, M-H, Thompson, FL, Sorokin, A, Normand, P, Dawyndt, P, Ehling-Schulz, M, Svensson, B, Sanchis, V, Nguyen-The, C, Heyndrickx, M, De Vos, P (B18) 2008; 10 Mogridge, J (B22) 2007; 4 Antonation, KS, Grützmacher, K, Dupke, S, Mabon, P, Zimmermann, F, Lankester, F, Peller, T, Feistner, A, Todd, A, Herbinger, I, de Nys, HM, Muyembe-Tamfun, J-J, Karhemere, S, Wittig, RM, Couacy-Hymann, E, Grunow, R, Calvignac-Spencer, S, Corbett, CR, Klee, SR, Leendertz, FH (B11) 2016; 10 Mills, E, Sullivan, E, Kovac, J (B4) 2022; 88 Kobayashi, A, Higashi, H, Shimada, T, Suzuki, S (B21) 2023; 5 Glasset, B, Herbin, S, Granier, SA, Cavalié, L, Lafeuille, E, Guérin, C, Ruimy, R, Casagrande-Magne, F, Levast, M, Chautemps, N, Decousser, J-W, Belotti, L, Pelloux, I, Robert, J, Brisabois, A, Ramarao, N (B3) 2018; 13 Hoffmaster, AR, Hill, KK, Gee, JE, Marston, CK, De, BK, Popovic, T, Sue, D, Wilkins, PP, Avashia, SB, Drumgoole, R, Helma, CH, Ticknor, LO, Okinaka, RT, Jackson, PJ (B13) 2006; 44 Akamatsu, R, Suzuki, M, Okinaka, K, Sasahara, T, Yamane, K, Suzuki, S, Fujikura, D, Furuta, Y, Ohnishi, N, Esaki, M, Shibayama, K, Higashi, H (B9) 2019; 25 Klee, SR, Brzuszkiewicz, EB, Nattermann, H, Brüggemann, H, Dupke, S, Wollherr, A, Franz, T, Pauli, G, Appel, B, Liebl, W, Couacy-Hymann, E, Boesch, C, Meyer, F-D, Leendertz, FH, Ellerbrok, H, Gottschalk, G, Grunow, R, Liesegang, H (B12) 2010; 5 |
| References_xml | – ident: e_1_3_4_4_2 doi: 10.1371/journal.pone.0194346 – ident: e_1_3_4_9_2 doi: 10.1007/s10096-010-1072-2 – ident: e_1_3_4_3_2 doi: 10.1016/B978-0-12-818619-0.00152-0 – ident: e_1_3_4_12_2 doi: 10.1371/journal.pntd.0004923 – ident: e_1_3_4_13_2 doi: 10.1371/journal.pone.0010986 – ident: e_1_3_4_16_2 doi: 10.1128/MRA.00766-23 – ident: e_1_3_4_2_2 doi: 10.1111/1574-6941.12110 – ident: e_1_3_4_20_2 doi: 10.1128/mBio.00034-20 – ident: e_1_3_4_10_2 doi: 10.3201/eid2505.171890 – ident: e_1_3_4_14_2 doi: 10.1128/JCM.00561-06 – ident: e_1_3_4_17_2 doi: 10.1111/j.1600-0463.2007.apm_630.x – ident: e_1_3_4_19_2 doi: 10.1111/j.1462-2920.2007.01495.x – ident: e_1_3_4_7_2 doi: 10.1016/j.jhin.2008.04.014 – ident: e_1_3_4_23_2 doi: 10.1016/j.ddmec.2007.12.002 – ident: e_1_3_4_5_2 doi: 10.1128/aem.02302-21 – ident: e_1_3_4_22_2 doi: 10.1016/j.infpip.2023.100272 – ident: e_1_3_4_21_2 doi: 10.3389/fmicb.2020.580691 – ident: e_1_3_4_6_2 doi: 10.1371/journal.pone.0235771 – ident: e_1_3_4_11_2 doi: 10.1186/s12941-015-0104-2 – ident: e_1_3_4_8_2 doi: 10.2169/internalmedicine.48.1885 – ident: e_1_3_4_15_2 doi: 10.1186/s12866-019-1678-1 – ident: e_1_3_4_18_2 doi: 10.1186/s13059-014-0524-x – volume: 13 year: 2018 ident: B3 article-title: Bacillus cereus, a serious cause of nosocomial infections: epidemiologic and genetic survey publication-title: PLoS One doi: 10.1371/journal.pone.0194346 – volume: 15 year: 2014 ident: B17 article-title: The harvest suite for rapid core-genome alignment and visualization of thousands of intraspecific microbial genomes publication-title: Genome Biol doi: 10.1186/s13059-014-0524-x – volume: 84 start-page: 433 year: 2013 end-page: 450 ident: B1 article-title: Diversity of Bacillus cereus group strains is reflected in their broad range of pathogenicity and diverse ecological lifestyles publication-title: FEMS Microbiol Ecol doi: 10.1111/1574-6941.12110 – volume: 19 year: 2019 ident: B14 article-title: Whole-genome sequence-based comparison and profiling of virulence-associated genes of Bacillus cereus group isolates from diverse sources in Japan publication-title: BMC Microbiol doi: 10.1186/s12866-019-1678-1 – volume: 13 year: 2024 ident: B15 article-title: Draft genome sequence including the capsule operon of a Bacillus cereus strain isolated from a patient with bacteremia in Japan publication-title: Microbiol Resour Announc doi: 10.1128/MRA.00766-23 – volume: 44 start-page: 3352 year: 2006 end-page: 3360 ident: B13 article-title: Characterization of Bacillus cereus isolates associated with fatal pneumonias: strains are closely related to Bacillus anthracis and harbor B. anthracis virulence genes publication-title: J Clin Microbiol doi: 10.1128/JCM.00561-06 – volume: 88 year: 2022 ident: B4 article-title: Comparative analysis of Bacillus cereus group isolates’ resistance using disk diffusion and broth microdilution and the correlation between antimicrobial resistance phenotypes and genotypes publication-title: Appl Environ Microbiol doi: 10.1128/aem.02302-21 – volume: 5 year: 2010 ident: B12 article-title: The genome of a Bacillus isolate causing anthrax in chimpanzees combines chromosomal properties of B. cereus with B. anthracis virulence plasmids publication-title: PLoS One doi: 10.1371/journal.pone.0010986 – volume: 10 year: 2016 ident: B11 article-title: Bacillus cereus biovar anthracis causing anthrax in sub-Saharan Africa-chromosomal monophyly and broad geographic distribution publication-title: PLoS Negl Trop Dis doi: 10.1371/journal.pntd.0004923 – volume: 115 start-page: 891 year: 2007 end-page: 899 ident: B16 article-title: Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci publication-title: APMIS doi: 10.1111/j.1600-0463.2007.apm_630.x – volume: 15 year: 2020 ident: B5 article-title: Ba813 harboring Bacillus cereus, genetically closely related to Bacillus anthracis, causing nosocomial bloodstream infection: bacterial virulence factors and clinical outcome publication-title: PLoS One doi: 10.1371/journal.pone.0235771 – volume: 25 start-page: 883 year: 2019 end-page: 890 ident: B9 article-title: Novel sequence type in Bacillus cereus strains associated with nosocomial infections and bacteremia, Japan publication-title: Emerg Infect Dis doi: 10.3201/eid2505.171890 – volume: 11 year: 2020 ident: B19 article-title: Proposal of a taxonomic nomenclature for the Bacillus cereus group which reconciles genomic definitions of bacterial species with clinical and industrial phenotypes publication-title: MBio doi: 10.1128/mBio.00034-20 – volume: 48 start-page: 791 year: 2009 end-page: 796 ident: B7 article-title: Nosocomial bacteremia caused by biofilm-forming Bacillus cereus and Bacillus thuringiensis publication-title: Intern Med doi: 10.2169/internalmedicine.48.1885 – volume: 30 start-page: 219 year: 2011 end-page: 226 ident: B8 article-title: Bacillus cereus bacteremia outbreak due to contaminated hospital linens publication-title: Eur J Clin Microbiol Infect Dis doi: 10.1007/s10096-010-1072-2 – volume: 4 start-page: 253 year: 2007 end-page: 258 ident: B22 article-title: Defensive strategies of Bacillus anthracis that promote a fatal disease publication-title: Drug Discov Today Dis Mech doi: 10.1016/j.ddmec.2007.12.002 – volume: 11 year: 2020 ident: B20 article-title: No assembly required: using BTyper3 to assess the congruency of a proposed taxonomic framework for the Bacillus cereus group with historical typing methods publication-title: Front Microbiol doi: 10.3389/fmicb.2020.580691 – volume: 14 year: 2015 ident: B10 article-title: Clinical characteristics and antimicrobial susceptibility of Bacillus cereus blood stream infections publication-title: Ann Clin Microbiol Antimicrob doi: 10.1186/s12941-015-0104-2 – volume: 5 start-page: 100272 year: 2023 ident: B21 article-title: Baseline and seasonal trends of Bacillus cereus and Bacillus subtilis from clinical samples in Japan publication-title: Infect Prev Pract doi: 10.1016/j.infpip.2023.100272 – start-page: 957 year: 2024 end-page: 986 ident: B2 article-title: The Bacillus cereus group publication-title: Molecular medical microbiology ;p In ;Elsevier ;London – volume: 10 start-page: 851 year: 2008 end-page: 865 ident: B18 article-title: Ecological diversification in the Bacillus cereus group publication-title: Environ Microbiol doi: 10.1111/j.1462-2920.2007.01495.x – volume: 69 start-page: 361 year: 2008 end-page: 367 ident: B6 article-title: Bacillus cereus nosocomial infection from reused towels in Japan publication-title: J Hosp Infect doi: 10.1016/j.jhin.2008.04.014 |
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| Snippet | This study provides novel insights into the genetic diversity and virulence potential of B. cereus strains causing bloodstream infections in a Japanese... catheter-related bloodstream infections (CRBSIs) are an increasing concern in Japanese hospitals. Although their clinical characteristics have been explored,... Bacillus cereus catheter-related bloodstream infections (CRBSIs) are an increasing concern in Japanese hospitals. Although their clinical characteristics have... Bacillus cereus catheter-related bloodstream infections (CRBSIs) are an increasing concern in Japanese hospitals. Although their clinical characteristics have... ABSTRACT Bacillus cereus catheter-related bloodstream infections (CRBSIs) are an increasing concern in Japanese hospitals. Although their clinical... |
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| SubjectTerms | Bacillus cereus - classification Bacillus cereus - genetics Bacillus cereus - isolation & purification Bacillus cereus - physiology Bacillus cereus group Bacteremia - microbiology Biofilms bloodstream infection Catheter-Related Infections - microbiology Cross Infection - microbiology epidemiology Genomics and Proteomics Gram-Positive Bacterial Infections - microbiology Humans Polymorphism, Single Nucleotide Research Article Whole Genome Sequencing whole-genome analysis |
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| Title | Genetic diversity and virulence of Bacillus cereus group isolates from bloodstream infections |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/39873504 https://journals.asm.org/doi/10.1128/spectrum.02407-24 https://www.proquest.com/docview/3160462900 https://pubmed.ncbi.nlm.nih.gov/PMC11878096 https://doaj.org/article/ad6c0ef2ca4f4ad9b9e11058b525fb24 |
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