Performance evaluation of cefoxitin screen test on two different automated antimicrobial susceptibility test systems: a comparative study

• Published in: Microbiology spectrum Vol. 12; no. 9; p. e0381523
• Main Authors: Yuceel-Timur, Ismail, Garner, Cherilyn D, Franklin, Simone, Hardy, Dwight J
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 03.09.2024
• Subjects: Anti-Bacterial Agents - pharmacology; Antimicrobial Chemotherapy; Bacterial Proteins - genetics; beta-Lactam Resistance; Cefoxitin - pharmacology; cefoxitin screen; Humans; methicillin-resistant Staphylococcus spp; Microbial Sensitivity Tests - methods; MRSA; Oxacillin - pharmacology; Penicillin-Binding Proteins - genetics; Phoenix; Research Article; Staphylococcal Infections - microbiology; Staphylococcus - drug effects; Staphylococcus - genetics; Staphylococcus spp; VITEK 2; VITEK 2; Staphylococcus spp; MRSA; BD Phoenix; Phoenix; oxacillin; cefoxitin (oxsf02n); cefoxitin screen; methicillin-resistant Staphylococcus spp
• ISSN: 2165-0497; 2165-0497
• DOI: 10.1128/spectrum.03815-23

Reliable detection of A and C-mediated beta-lactam resistance using automated antimicrobial susceptibility test systems is critical for patient care. The aim of this study was to compare the performance of the new cefoxitin screen test (oxsf02n) on the Vitek 2 card (Vitek 2) and BD Phoenix PMC-100 Gram-Positive AST Panel (Phoenix) against the reference method for the detection of A (and C)-mediated beta-lactam resistance. Two hundred fifty clinical fresh and stock spp. isolates were included. There were 120 A-positive, 10 C-positive, and 120 A and C-negative isolates. Cefoxitin screen and oxacillin tests were performed on Vitek 2 and Phoenix and by their respective reference methods (disk diffusion for the cefoxitin screen test and broth microdilution for oxacillin) for all isolates. PCR testing was also performed to confirm the presence of A and/or C genes. Results from each system were compared to the reference methods. Statistical hypotheses were evaluated both for Vitek 2 compared to the reference methods and Vitek 2 compared to the Phoenix. Compared to the reference method, the Vitek 2 cefoxitin screen test had 100% sensitivity/98% specificity and the Phoenix cefoxitin screen test had 84% sensitivity/100% specificity for the detection of A (and C)-mediated beta-lactam resistance. When the oxacillin test was combined with the cefoxitin screen for Vitek 2, the sensitivity and specificity were unchanged. However, when the oxacillin and cefoxitin screen tests were combined for the Phoenix, the sensitivity increased to 100% and the specificity remained unchanged (100%). When considering cefoxitin alone, the Vitek 2 screen test showed a higher sensitivity than the Phoenix for the detection of A and C-mediated beta-lactam resistance. However, currently, both systems use a combination of the cefoxitin and oxacillin tests to interpret the final result, and both reached a high level of performance when cefoxitin and oxacillin results were combined.IMPORTANCEThis research marks the inaugural evaluation of the revamped cefoxitin screen test version in Vitek 2, juxtaposing it against reference methods and a primary competitor BD Phoenix.