Bioluminescence Imaging of Urokinase-Type Plasminogen Activator Activity in Vitro and in Tumors
Urokinase-type plasminogen activator (uPA) is a cell-secreted serine protease and plays a significant role in numerous biological processes. Overexpression of uPA has been proved to be relevant to some malignant tumors as well as poor prognosis. However, bioluminescence (BL) probes for selectively s...
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| Published in | Analytical chemistry (Washington) Vol. 93; no. 29; pp. 9970 - 9973 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Washington
American Chemical Society
27.07.2021
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0003-2700 1520-6882 1520-6882 |
| DOI | 10.1021/acs.analchem.1c02499 |
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| Abstract | Urokinase-type plasminogen activator (uPA) is a cell-secreted serine protease and plays a significant role in numerous biological processes. Overexpression of uPA has been proved to be relevant to some malignant tumors as well as poor prognosis. However, bioluminescence (BL) probes for selectively sensing uPA activity have not been reported up to now. Herein, we designed a BL probe, GGR-AmLuc, to detect uPA in vitro and sense uPA both inside cells and in tumors. In vitro studies demonstrated that GGR-AmLuc was able to selectively detect uPA with a limit of detection (LOD) of 1.37 μg/L. Moreover, GGR-AmLuc was successfully applied to image uPA in living subjects with excellent sensitivity. We anticipate that probe GGR-AmLuc could be applied for highly sensitive diagnosis of cancers overexpressing uPA and provide guidance for cancer treatment in the near future. |
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| AbstractList | Urokinase-type plasminogen activator (uPA) is a cell-secreted serine protease and plays a significant role in numerous biological processes. Overexpression of uPA has been proved to be relevant to some malignant tumors as well as poor prognosis. However, bioluminescence (BL) probes for selectively sensing uPA activity have not been reported up to now. Herein, we designed a BL probe, GGR-AmLuc, to detect uPA in vitro and sense uPA both inside cells and in tumors. In vitro studies demonstrated that GGR-AmLuc was able to selectively detect uPA with a limit of detection (LOD) of 1.37 μg/L. Moreover, GGR-AmLuc was successfully applied to image uPA in living subjects with excellent sensitivity. We anticipate that probe GGR-AmLuc could be applied for highly sensitive diagnosis of cancers overexpressing uPA and provide guidance for cancer treatment in the near future. Urokinase-type plasminogen activator (uPA) is a cell-secreted serine protease and plays a significant role in numerous biological processes. Overexpression of uPA has been proved to be relevant to some malignant tumors as well as poor prognosis. However, bioluminescence (BL) probes for selectively sensing uPA activity have not been reported up to now. Herein, we designed a BL probe, GGR-AmLuc, to detect uPA in vitro and sense uPA both inside cells and in tumors. In vitro studies demonstrated that GGR-AmLuc was able to selectively detect uPA with a limit of detection (LOD) of 1.37 μg/L. Moreover, GGR-AmLuc was successfully applied to image uPA in living subjects with excellent sensitivity. We anticipate that probe GGR-AmLuc could be applied for highly sensitive diagnosis of cancers overexpressing uPA and provide guidance for cancer treatment in the near future.Urokinase-type plasminogen activator (uPA) is a cell-secreted serine protease and plays a significant role in numerous biological processes. Overexpression of uPA has been proved to be relevant to some malignant tumors as well as poor prognosis. However, bioluminescence (BL) probes for selectively sensing uPA activity have not been reported up to now. Herein, we designed a BL probe, GGR-AmLuc, to detect uPA in vitro and sense uPA both inside cells and in tumors. In vitro studies demonstrated that GGR-AmLuc was able to selectively detect uPA with a limit of detection (LOD) of 1.37 μg/L. Moreover, GGR-AmLuc was successfully applied to image uPA in living subjects with excellent sensitivity. We anticipate that probe GGR-AmLuc could be applied for highly sensitive diagnosis of cancers overexpressing uPA and provide guidance for cancer treatment in the near future. |
| Author | Shen, Yang Liang, Gaolin Chen, Yinglu Zhang, Tong Wang, Chenchen Wu, Chengfan Hua, Yue |
| AuthorAffiliation | Southeast University School of Life Sciences Hefei National Laboratory of Physical Sciences at Microscale, Department of Chemistry Department of Obstetrics and Gynaecology, Zhongda Hospital, School of Medicine State Key Laboratory of Bioelectronics, School of Biological Sciences and Medical Engineering |
| AuthorAffiliation_xml | – name: State Key Laboratory of Bioelectronics, School of Biological Sciences and Medical Engineering – name: Department of Obstetrics and Gynaecology, Zhongda Hospital, School of Medicine – name: Southeast University – name: School of Life Sciences – name: Hefei National Laboratory of Physical Sciences at Microscale, Department of Chemistry |
| Author_xml | – sequence: 1 givenname: Yinglu surname: Chen fullname: Chen, Yinglu organization: Hefei National Laboratory of Physical Sciences at Microscale, Department of Chemistry – sequence: 2 givenname: Chengfan surname: Wu fullname: Wu, Chengfan organization: Hefei National Laboratory of Physical Sciences at Microscale, Department of Chemistry – sequence: 3 givenname: Chenchen surname: Wang fullname: Wang, Chenchen organization: Hefei National Laboratory of Physical Sciences at Microscale, Department of Chemistry – sequence: 4 givenname: Tong surname: Zhang fullname: Zhang, Tong organization: School of Life Sciences – sequence: 5 givenname: Yue surname: Hua fullname: Hua, Yue organization: Southeast University – sequence: 6 givenname: Yang surname: Shen fullname: Shen, Yang email: shenyang@seu.edu.cn organization: Southeast University – sequence: 7 givenname: Gaolin orcidid: 0000-0002-6159-9999 surname: Liang fullname: Liang, Gaolin email: gliang@ustc.edu.cn organization: Southeast University |
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| SubjectTerms | Analytical chemistry Biological activity Bioluminescence Cancer cancer therapy Chemistry detection limit prognosis Serine Serine proteinase Thrombolytic drugs Tumors U-Plasminogen activator Urokinase |
| Title | Bioluminescence Imaging of Urokinase-Type Plasminogen Activator Activity in Vitro and in Tumors |
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