Hydrodynamic characterization of the photoaffinity-labeled insulin receptor solubilized in Triton X-100

• Published in: Biochemistry (Easton) Vol. 20; no. 14; pp. 4156 - 4161
• Main Authors: Baron, Michael D, Wisher, Martin H, Thamm, Peter M, Saunders, Derek J, Brandenburg, Dietrich, Soenksen, Peter H
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.07.1981
• Subjects: Affinity Labels; Animals; Azides; Cell Membrane - metabolism; Detergents; insulin; Insulin - analogs & derivatives; Insulin - metabolism; Kinetics; liver; Liver - metabolism; Macromolecular Substances; Molecular Weight; Octoxynol; physicochemical properties; plasma membranes; Polyethylene Glycols; purification; Rats; Receptor, Insulin - isolation & purification; Receptor, Insulin - metabolism

The insulin receptor in isolated rat liver plasma membranes was covalently labeled with the photoreactive insulin analogue NB-29-[(4-azido-2-nitrophenyl)acetyl]insulin and solubilized with the nondenaturing detergent Triton X-100. The resulting protein-detergent complex was characterized by gel filt...