Circular dichroism study of the unfolding-refolding of a cardiotoxin from Taiwan cobra (Naja naja atra) venom

• Published in: Biochemistry (Easton) Vol. 24; no. 20; pp. 5678 - 5685
• Main Authors: Galat, Andrzej, Yang, Chenchung, Blout, Elkan R
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.09.1985
• Subjects: Amino Acid Sequence; Biological and medical sciences; Circular Dichroism; Cobra Cardiotoxin Proteins - metabolism; Conformational dynamics in molecular biology; Disulfides - analysis; Elapid Venoms - metabolism; Fundamental and applied biological sciences. Psychology; Models, Molecular; Molecular biophysics; Protein Conformation; Proteins; Toxin; Vertebrata; Refolding; Venom; Unfolding; Reptilia; Circular dichroism; Ophidia; Conformational transition

Circular dichroism spectroscopy has been used to study the unfolding-refolding process of a cardiotoxin from Taiwan cobra (Naja naja atra) venom upon addition of fluoroalcohols or sodium dodecyl sulfate (SDS) to its aqueous solution. In these experiments, the disulfide bridges remained intact. The u...