A Complex Standard for Protein Identification, Designed by Evolution

• Published in: Journal of proteome research Vol. 11; no. 10; pp. 5065 - 5071
• Main Authors: Vaudel, Marc, Burkhart, Julia M, Breiter, Daniela, Zahedi, René P, Sickmann, Albert, Martens, Lennart
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 05.10.2012
• Subjects: Algorithms; Animals; Archaeal Proteins - chemistry; Chromatography, Reverse-Phase - standards; Complex Mixtures - chemistry; Evolution, Molecular; False Positive Reactions; Humans; Peptide Mapping - methods; Peptide Mapping - standards; peptides; proteome; proteomics; Pyrococcus furiosus; Pyrococcus furiosus - chemistry; Reference Standards; Tandem Mass Spectrometry - standards; Workflow; Pyrococcus furiosus; peptide identification; protein identification; bioinformatics

Shotgun proteomic investigations rely on the algorithmic assignment of mass spectra to peptides. The quality of these matches is therefore a cornerstone in the analysis and has been the subject of numerous recent developments. In order to establish the benefits of novel algorithms, they are applied...