Detection of neocarzinostatin chromophore-deoxyribose adducts as exonuclease-resistant sites in defined-sequence DNA

• Published in: Biochemistry (Easton) Vol. 24; no. 15; pp. 4035 - 4040
• Main Authors: Povirk, Lawrence F., Goldberg, Irving H.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.07.1985
• Subjects: Animals; Antibiotics, Antineoplastic - analysis; Antineoplastic agents; Base Sequence; Biological and medical sciences; Cattle; Deoxyribose - analysis; DNA; DNA Restriction Enzymes; Enediynes; Exodeoxyribonucleases - metabolism; General aspects; Kinetics; Medical sciences; Pharmacology. Drug treatments; Substrate Specificity; Thymus Gland; Zinostatin - analogs & derivatives; Zinostatin - analysis; Antineoplastic agent; Molecular interaction; DNA; Molecular adduct; Chromophore

A 5'-end-labeled DNA restriction fragment was treated with the nonprotein chromophore of neocarzinostatin under anoxia in the presence of dithiothreitol, conditions known to maximize formation of chromophore-deoxyribose adducts. Under conditions where unmodified DNA was digested to completion,...