Dependence of Purple Membrane Bump Curvature on pH and Ionic Strength Analyzed Using Atomic Force Microscopy Combined with Solvent Exchange

• Published in: The journal of physical chemistry. B Vol. 118; no. 31; pp. 9322 - 9328
• Main Authors: Yokoyama, Yasunori, Yamada, Kosuke, Higashi, Yosuke, Ozaki, Satoshi, Wang, Haorang, Koito, Naoki, Watanabe, Naoya, Sonoyama, Masashi, Mitaku, Shigeki
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 07.08.2014
• Subjects: amino acids; Atomic force microscopy; Calcium - metabolism; calcium chloride; Calcium Chloride - chemistry; Curvature; electrostatic interactions; Formations; Halobacterium salinarum; Hydrogen-Ion Concentration; ionic strength; Ions - chemistry; Linear Models; lipids; Mathematical analysis; membrane proteins; Membranes; Microscopy, Atomic Force; Models, Chemical; Osmolar Concentration; physical chemistry; potassium chloride; Potassium Chloride - chemistry; Purple Membrane - chemistry; Self assembly; Solvents; Static Electricity; topography; Water - chemistry

Purple membrane (PM), which is a membrane patch formed by the self-assembly of the membrane protein bacteriorhodopsin (bR) with archaeal lipids, is a good subject for studying the mechanism for the supramolecular structural formation of membrane proteins. Several studies have suggested that PM is no...