High-Antibody-Producing Chinese Hamster Ovary Cells Up-Regulate Intracellular Protein Transport and Glutathione Synthesis

• Published in: Journal of proteome research Vol. 14; no. 2; pp. 609 - 618
• Main Authors: Orellana, Camila A, Marcellin, Esteban, Schulz, Benjamin L, Nouwens, Amanda S, Gray, Peter P, Nielsen, Lars K
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 06.02.2015
• Subjects: Animals; Antibodies, Monoclonal - biosynthesis; CHO Cells; Cricetinae; Cricetulus; Female; Glutathione - biosynthesis; Ovary - immunology; Protein Transport; Up-Regulation; iTRAQ; SWATH; CHO cells; glutathione; quantitative proteomics; monoclonal antibodies

Chinese hamster ovary (CHO) cells are the preferred production host for therapeutic monoclonal antibodies (mAb) due to their ability to perform post-translational modifications and their successful approval history. The completion of the genome sequence for CHO cells has reignited interest in using...