Biophysical Characteristics of Cholera Toxin and Escherichia coli Heat-Labile Enterotoxin Structure and Chemistry Lead to Differential Toxicity

• Published in: The journal of physical chemistry. B Vol. 119; no. 3; pp. 1048 - 1061
• Main Authors: Craft, John W, Shen, Tsai-wei, Brier, Lindsey M, Briggs, James M
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 22.01.2015
• Subjects: Amino acids; Aspartic acid; Backbone; Biophysical Phenomena; Cholera Toxin - chemistry; Cholera Toxin - metabolism; Cholera Toxin - toxicity; Escherichia coli; Hot Temperature; Hydrogen Bonding; Molecular Dynamics Simulation; Porosity; Protein Conformation; Similarity; Solvents - chemistry; Structure-Activity Relationship; Surface Properties; Toxicity; Toxins; Vibrio cholerae; Water - chemistry

The biophysical chemistry of macromolecular complexes confer their functional characteristics. We investigate the mechanisms that make the AB5 holotoxin of Vibrio cholerae (CT) a significantly more pathogenic molecule than the enterotoxin of Escherichia coli (LT) with which it shares 88% similarity...