High Resolution Printing of DNA Feature on Poly(methyl methacrylate) Substrates Using Supramolecular Nano-Stamping

• Published in: Journal of the American Chemical Society Vol. 127; no. 48; pp. 16774 - 16775
• Main Authors: Yu, Arum Amy, Savas, Tim, Cabrini, Stefano, diFabrizio, Enzo, Smith, Henry I, Stellacci, Francesco
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 07.12.2005
• Subjects: Biological and medical sciences; Biotechnology; DNA, Single-Stranded - chemistry; Fundamental and applied biological sciences. Psychology; General aspects; Immobilization techniques; Methods. Procedures. Technologies; Microscopy, Atomic Force; Nanotechnology - methods; Polymethyl Methacrylate - chemistry; Atomic force microscopy; Patterning; Functionalization; Molecular wires; Supramolecular structure; Methyl methacrylate polymer; Confocal microscopy; Nucleic acid; Fluorescence microscopy; Surface treatment
• ISSN: 0002-7863; 1520-5126
• DOI: 10.1021/ja055762e

In recent years, a large number of devices based on organic and biological materials have been developed. To scale-up the production of these systems to industrially acceptable standards, there is a need to develop soft-material stamping approaches with the needed resolution, complexity, and versatility. We have recently developed a DNA-based stamping method (supramolecular nano-stamping, SuNS) that has superior resolution and can print multiple molecules at the same time. A similar technique was independently developed by Crooks and co-workers. Here we show that SuNS can be used to efficiently print DNA features on a polymeric substrate (poly(methyl methacrylate), PMMA) with a 40 nm point resolution and a coverage that exceeds 100 μm2. The stamped PMMA substrate was also used as a master to print on a gold substrate. With PMMA being optically clear and electrically insulating, future applications of SuNS to print DNA micro- and nanoarrays are envisioned.