Examination of Thermal Unfolding and Aggregation Profiles of a Series of Developable Therapeutic Monoclonal Antibodies

• Published in: Molecular pharmaceutics Vol. 12; no. 4; pp. 1005 - 1017
• Main Authors: Brader, Mark L, Estey, Tia, Bai, Shujun, Alston, Roy W, Lucas, Karin K, Lantz, Steven, Landsman, Pavel, Maloney, Kevin M
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 06.04.2015
• Subjects: Antibodies, Monoclonal - chemistry; Calorimetry, Differential Scanning; Chemistry, Pharmaceutical; Chromatography, High Pressure Liquid; Drug Stability; Humans; Immunoglobulin G - chemistry; Light; Protein Conformation; Protein Denaturation; Protein Folding; Protein Stability; Scattering, Radiation; Spectrometry, Fluorescence; Temperature; antibody therapeutics; protein formulation; thermal denaturation; developability; developable; protein aggregation; high-throughput screening; stability

Screening for pharmaceutically viable stability from measurements of thermally induced protein unfolding and short-term accelerated stress underpins much molecule design, selection, and formulation in the pharmaceutical biotechnology industry. However, the interrelationships among intrinsic protein...