Expression and Stereochemical and Isotope Effect Studies of Active 4-Oxalocrotonate Decarboxylase
4-Oxalocrotonate decarboxylase (4-OD) and vinylpyruvate hydratase (VPH) from Pseudomonas putida mt-2 form a complex that converts 2-oxo-3-hexenedioate to 2-oxo-4-hydroxypentanoate in the catechol meta fission pathway. To facilitate mechanistic and structural studies of the complex, the two enzymes h...
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Published in | Biochemistry (Easton) Vol. 39; no. 4; pp. 718 - 726 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Chemical Society
01.02.2000
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Subjects | |
Online Access | Get full text |
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