Multifunctional Nanobeads Based on Quantum Dots and Magnetic Nanoparticles: Synthesis and Cancer Cell Targeting and Sorting

• Published in: ACS nano Vol. 5; no. 2; pp. 1109 - 1121
• Main Authors: Di Corato, Riccardo, Bigall, Nadja C, Ragusa, Andrea, Dorfs, Dirk, Genovese, Alessandro, Marotta, Roberto, Manna, Liberato, Pellegrino, Teresa
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 22.02.2011
• Subjects: Biological Transport; Cell Separation - methods; Ethylamines - chemistry; Fluorescent Dyes - chemistry; Folic Acid - chemistry; Humans; KB Cells; Magnetics; Microscopy, Confocal; Neoplasms - metabolism; Neoplasms - pathology; Quantum Dots; Spectrometry, Fluorescence; Surface Properties; quantum dots; magnetic clusters; magnetic−fluorescent nanostructures; magnetic nanoparticles; cell sorting; tumor labeling
• ISSN: 1936-0851; 1936-086X
• DOI: 10.1021/nn102761t

Trifunctional polymer nanobeads are prepared by destabilization of a mixture of magnetic nanoparticles, quantum dots, and an amphiphilic polymer, followed by functionalization of the bead surface with folic acid molecules. The distribution of the nanoparticles within the nanobeads can be tuned using either acetonitrile or water as destabilizing solvent. The luminescence of the resulting beads can be tuned by varying the ratio of quantum dots per magnetic nanoparticles. The application of an external magnetic field (such as a small static magnet of 0.3 T) to the magnetic−fluorescent nanobeads allows the quantitative accumulation of the beads within a few hours depending on the total size of the beads. Furthermore, specific targeting of cancer cells overexpressing folate receptors is achieved thanks to the folic acid decorating the surface of the as-synthesized nanobeads. Folate receptor mediated cellular uptake of the folic acid-functionalized nanobeads is proven via both confocal imaging and transmission electron microscopy characterization. Cell sorting experiments performed with trifunctional nanobeads show quantitative recovering of targeted cells even when they are present at low percentage (up to 1%).