Rational Domain Swaps Decipher Programming in Fungal Highly Reducing Polyketide Synthases and Resurrect an Extinct Metabolite

• Published in: Journal of the American Chemical Society Vol. 133; no. 41; pp. 16635 - 16641
• Main Authors: Fisch, Katja M, Bakeer, Walid, Yakasai, Ahmed A, Song, Zhongshu, Pedrick, Jennifer, Wasil, Zahida, Bailey, Andrew M, Lazarus, Colin M, Simpson, Thomas J, Cox, Russell J
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 19.10.2011; Amer Chemical Soc
• Subjects: Aspergillus oryzae - enzymology; Chemistry; Chemistry, Multidisciplinary; Models, Molecular; Oxidation-Reduction; Physical Sciences; Polyketide Synthases - chemistry; Polyketide Synthases - metabolism; Pyridones - chemistry; Pyridones - metabolism; Science & Technology; KETOSYNTHASE DOMAIN; 2-PYRIDONE TENELLIN; FATTY-ACID SYNTHASE; BEAUVERIA-BASSIANA; PROTEINS; BIOSYNTHESIS

The mechanism of programming of iterative highly reducing polyketide synthases remains one of the key unsolved problems of secondary metabolism. We conducted rational domain swaps between the polyketide synthases encoding the biosynthesis of the closely related compounds tenellin and desmethylbassia...