Rational Domain Swaps Decipher Programming in Fungal Highly Reducing Polyketide Synthases and Resurrect an Extinct Metabolite
The mechanism of programming of iterative highly reducing polyketide synthases remains one of the key unsolved problems of secondary metabolism. We conducted rational domain swaps between the polyketide synthases encoding the biosynthesis of the closely related compounds tenellin and desmethylbassia...
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| Published in | Journal of the American Chemical Society Vol. 133; no. 41; pp. 16635 - 16641 |
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| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
WASHINGTON
American Chemical Society
19.10.2011
Amer Chemical Soc |
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| Abstract | The mechanism of programming of iterative highly reducing polyketide synthases remains one of the key unsolved problems of secondary metabolism. We conducted rational domain swaps between the polyketide synthases encoding the biosynthesis of the closely related compounds tenellin and desmethylbassianin. Expression of the hybrid synthetases in Aspergillus oryzae led to the production of reprogrammed compounds in which the changes to the methylation pattern and chain length could be mapped to the domain swaps. These experiments reveal for the first time the origin of programming in these systems. Domain swaps combined with coexpression of two cytochrome P450 encoding genes from the tenellin biosynthetic gene cluster led to the resurrection of the extinct metabolite bassianin. |
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| AbstractList | The mechanism of programming of iterative highly reducing polyketide synthases remains one of the key unsolved problems of secondary metabolism. We conducted rational domain swaps between the polyketide synthases encoding the biosynthesis of the closely related compounds tenellin and desmethylbassianin. Expression of the hybrid synthetases in Aspergillus oryzae led to the production of reprogrammed compounds in which the changes to the methylation pattern and chain length could be mapped to the domain swaps. These experiments reveal for the first time the origin of programming in these systems. Domain swaps combined with coexpression of two cytochrome P450 encoding genes from the tenellin biosynthetic gene cluster led to the resurrection of the extinct metabolite bassianin. |
| Author | Bakeer, Walid Cox, Russell J Fisch, Katja M Lazarus, Colin M Song, Zhongshu Pedrick, Jennifer Bailey, Andrew M Simpson, Thomas J Wasil, Zahida Yakasai, Ahmed A |
| AuthorAffiliation | University of Bristol Beni Suef University |
| AuthorAffiliation_xml | – name: Beni Suef University – name: University of Bristol |
| Author_xml | – sequence: 1 givenname: Katja M surname: Fisch fullname: Fisch, Katja M – sequence: 2 givenname: Walid surname: Bakeer fullname: Bakeer, Walid – sequence: 3 givenname: Ahmed A surname: Yakasai fullname: Yakasai, Ahmed A – sequence: 4 givenname: Zhongshu surname: Song fullname: Song, Zhongshu – sequence: 5 givenname: Jennifer surname: Pedrick fullname: Pedrick, Jennifer – sequence: 6 givenname: Zahida surname: Wasil fullname: Wasil, Zahida – sequence: 7 givenname: Andrew M surname: Bailey fullname: Bailey, Andrew M – sequence: 8 givenname: Colin M surname: Lazarus fullname: Lazarus, Colin M – sequence: 9 givenname: Thomas J surname: Simpson fullname: Simpson, Thomas J email: r.j.cox@bris.ac.uk, tom.simpson@bris.ac.uk – sequence: 10 givenname: Russell J surname: Cox fullname: Cox, Russell J email: r.j.cox@bris.ac.uk, tom.simpson@bris.ac.uk |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/21899331$$D View this record in MEDLINE/PubMed |
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| Keywords | KETOSYNTHASE DOMAIN 2-PYRIDONE TENELLIN FATTY-ACID SYNTHASE BEAUVERIA-BASSIANA PROTEINS BIOSYNTHESIS |
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| Snippet | The mechanism of programming of iterative highly reducing polyketide synthases remains one of the key unsolved problems of secondary metabolism. We conducted... |
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| SubjectTerms | Aspergillus oryzae - enzymology Chemistry Chemistry, Multidisciplinary Models, Molecular Oxidation-Reduction Physical Sciences Polyketide Synthases - chemistry Polyketide Synthases - metabolism Pyridones - chemistry Pyridones - metabolism Science & Technology |
| Title | Rational Domain Swaps Decipher Programming in Fungal Highly Reducing Polyketide Synthases and Resurrect an Extinct Metabolite |
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