Initial Disulfide Formation Steps in the Folding of an ω-Conotoxin

• Published in: Biochemistry (Easton) Vol. 41; no. 10; pp. 3507 - 3519
• Main Authors: Price-Carter, Marian, Bulaj, Grzegorz, Goldenberg, David P
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 12.03.2002
• Subjects: Amino Acid Sequence; Conus; Cysteine - chemistry; Disulfides - metabolism; Kinetics; Marine; Molecular Sequence Data; omega -Conotoxin; omega-Conotoxins - chemistry; omega-Conotoxins - metabolism; Peptide Mapping; Protein Folding

To determine whether the native disulfides of ω-conotoxins are preferentially stabilized early in the folding of these small proteins, the rates and equilibria for disulfide formation were measured for three analogues of ω-conotoxin MVIIA. In each analogue, one of the three pairs of disulfide-bonded...