Quantitative Analysis of the Human Spindle Phosphoproteome at Distinct Mitotic Stages

• Published in: Journal of proteome research Vol. 8; no. 10; pp. 4553 - 4563
• Main Authors: Malik, Rainer, Lenobel, René, Santamaria, Anna, Ries, Albert, Nigg, Erich A, Körner, Roman
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.10.2009
• Subjects: Cluster Analysis; Consensus Sequence; Culture Media; HeLa Cells; Humans; Mitosis - physiology; Phosphoproteins - analysis; Phosphorylation; Phosphoserine; Phosphothreonine; Phosphotransferases; Proteome - analysis; Reproducibility of Results; Spindle Apparatus - metabolism; Time Factors; signaling; mitosis; spindle; phosphorylation; kinase consensus

During mitosis, phosphorylation of spindle associated proteins is a key regulatory mechanism for spindle formation, mitotic progression, and cytokinesis. In the recent past, mass spectrometry has been applied successfully to identify spindle proteomes and phosphoproteomes, but did not address their...